2026-06-14T06:44:10Zhttps://repisalud.isciii.es/rest/oai/requestoai:repisalud.isciii.es:20.500.12105/77032025-06-17T08:00:32Zcom_20.500.12105_2052com_20.500.12105_2051col_20.500.12105_19609
Repisalud
author
Rodriguez-Tudela, Juan Luis
author
Diaz-Guerra, Teresa M.
author
Mellado, Emilia
author
Cano, Virginia
author
Tapia, Cecilia
author
Perkins, Alexander
author
Gomez-Lopez, Alicia
author
Rodero, Laura
author
Cuenca-Estrella, Manuel
2019-05-31T10:47:55Z
2019-05-31T10:47:55Z
2005-10
Antimicrob Agents Chemother. 2005;49(10):4026-34.
10.1128/AAC.49.10.4026-4034.2005
0066-4804
Antimicrobial agents and chemotherapy
16189076
http://hdl.handle.net/20.500.12105/7703
The physiological patterns, the sequence polymorphisms of the internal transcriber spacer (ITS), and intergenic spacer regions (IGS) of the rRNA genes and the antifungal susceptibility profile were evaluated for their ability to identify Trichosporon spp. and their specificity for the identification of 49 clinical isolates of Trichosporon spp. Morphological and biochemical methodologies were unable to differentiate among the Trichosporon species. ITS sequencing was also unable to differentiate several species. However, IGS1 sequencing unambiguously identified all Trichosporon isolates. Following the results of DNA-based identification, Trichosporon asahii was the species most frequently isolated from deep sites (15 of 25 strains; 60%). In the main, other Trichosporon species were recovered from cutaneous samples. The majority of T. asahii, T. faecale, and T. coremiiforme clinical isolates exhibited resistance in vitro to amphotericin B, with geometric mean (GM) MICs >4 mug/ml. The other species of Trichosporon did not show high MICs of amphotericin B, and GM MICs were <1 mug/ml. Azole agents were active in vitro against the majority of clinical strains. The most potent compound in vitro was voriconazole, with a GM MIC </=0.14 mug/ml. The sequencing of IGS correctly identified Trichosporon isolates; however, this technique is not available in many clinical laboratories, and strains should be dispatched to reference centers where these complex methods are available. Therefore, it seems to be more practical to perform antifungal susceptibility testing of all isolates belonging to Trichosporon spp., since correct identification could take several weeks, delaying the indication of an antifungal agent which exhibits activity against the infectious strain.
eng
Susceptibility patterns and molecular identification of Trichosporon species
research article
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SusceptibilityPatternsMolecularIdentification_2005.pdf
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Tablas_Susceptibility patterns and molecular identification_2005.pdf
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SusceptibilityPatternsMolecularIdentification_2005.pdf.txt
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Tablas_Susceptibility patterns and molecular identification_2005.pdf.txt