2026-04-29T04:09:39Zhttps://repisalud.isciii.es/rest/oai/requestoai:repisalud.isciii.es:20.500.12105/76952025-06-09T14:56:16Zcom_20.500.12105_2052com_20.500.12105_2051col_20.500.12105_19609
Repisalud
author
Borderia, Antonio V.
author
Lorenzo-Redondo, Ramon
author
Pernas, Maria
author
Casado, Concepcion
author
Alvaro-Cifuentes, Tamara
author
Domingo, Esteban
author
Lopez-Galindez, Luis Cecilio
funder
Fundación para la Investigación y la Prevención del Sida en España
funder
Ministerio de Ciencia e Innovación (España)
funder
Fundación Ramón Areces
funder
Instituto de Salud Carlos III
2019-05-29T10:37:23Z
2019-05-29T10:37:23Z
2010-04
PLoS One. 2010;5(4):e10319.
1932-6203
http://hdl.handle.net/20.500.12105/7695
20436678
10.1371/journal.pone.0010319
1932-6203
PLoS ONE
BACKGROUND: Fitness recovery of HIV-1 "in vitro" was studied using viral clones that had their fitness decreased as a result of plaque-to-plaque passages. PRINCIPAL FINDINGS: After ten large population passages, the viral populations showed an average increase of fitness, although with wide variations among clones. While 5 clones showed significant fitness increases, 3 clones showed increases that were only marginally significant (p<0.1), and 4 clones did not show any change. Fitness recovery was not accompanied by an increase in p24 production, but was associated with an increase in viral titer. Few mutations (an average of 2 mutations per genome) were detected in the consensus nucleotide sequence of the entire genome in all viral populations. Five of the populations did not fix any mutation, and three of them displayed marginally significant fitness increases, illustrating that fitness recovery can occur without detectable alterations of the consensus genomic sequence. The investigation of other possible viral factors associated with the initial steps of fitness recovery, showed that viral quasispecies heterogeneity increased between the initial clones and the passaged populations. A direct statistical correlation between viral heterogeneity and viral fitness was obtained. CONCLUSIONS: Thus, the initial fitness recovery of debilitated HIV-1 clones was mediated by an increase in quasispecies heterogeneity. This observation, together with the invariance of the consensus sequence despite fitness increases demonstrates the relevance of quasispecies heterogeneity in the evolution of HIV-1 in cell culture.
eng
Initial Fitness Recovery of HIV-1 Is Associated with Quasispecies Heterogeneity and Can Occur without Modifications in the Consensus Sequence
research article
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URL
https://repisalud.isciii.es/bitstreams/07c688b5-d1e2-4a12-ab8e-4ac7f4120cb1/download
File
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application/pdf
InitialFitnessRecoveryOf_2010.pdf
URL
https://repisalud.isciii.es/bitstreams/96c1c759-97b0-4964-a6d7-99c71546f5a6/download
File
MD5
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text/plain
InitialFitnessRecoveryOf_2010.pdf.txt