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oai:repisalud.isciii.es:20.500.12105/76722024-09-27T10:05:26Zcom_20.500.12105_19604com_20.500.12105_2051col_20.500.12105_19607

00925njm 22002777a 4500 dc Fresno, Cristóbal author Llera, Andrea S author Girotti, María R author Valacco, María P author Lopez, Juan Antonio author Podhajcer, Osvaldo L author Balzarini, Mónica G author Prada, Federico author Fernández, Elmer A author 2012-02 Set enrichment analysis (SEA) is used to identify enriched biological categories/terms within high-throughput differential expression experiments. This is done by evaluating the proportion of differentially expressed genes against a background reference (BR). However, the choice of the "appropriate" BR is a perplexing problem and results will depend on it. Here, a visualization procedure that integrates results from several BRs and a stability analysis of enriched terms is presented as a tool to aid SEA. The multi-reference contrast method (MRCM) combines results from multiple BRs in a unique picture. The application of the proposed method was illustrated in one proteomic and three microarray experiments. The MRCM facilitates the exploration task involved in ontology analysis on proteomic/genomic experiments, where consensus terms were found to validate main experimental hypothesis. The use of more than one reference may provide new biological insights. The tool automatically highlights non-consensus terms assisting SEA. Comput Biol Med. 2012; 42(2):188-94 10.1016/j.compbiomed.2011.11.007 1879-0534 00104825 Computers in biology and medicine 22226646 http://hdl.handle.net/20.500.12105/7672 The multi-reference contrast method: facilitating set enrichment analysis