2026-07-21T20:18:26Zhttps://repisalud.isciii.es/rest/oai/requestoai:repisalud.isciii.es:20.500.12105/76712024-09-27T10:13:24Zcom_20.500.12105_19604com_20.500.12105_2051col_20.500.12105_19607
Repisalud
author
Martinez-Pinna, Roxana
author
Lopez, Juan Antonio
author
Ramos-Mozo, Priscila
author
Blanco-Colio, Luis M
author
Camafeita, Emilio
author
Calvo, Enrique
author
Meilhac, Olivier
author
Michel, Jean Baptiste
author
Egido, Jesús
author
Martin-Ventura, José Luis
funder
Ministerio de Ciencia e Innovación (España)
funder
Ministerio de Sanidad y Consumo (España)
funder
Instituto de Salud Carlos III
funder
Unión Europea. Comisión Europea
funder
Fundación ProCNIC
2019-05-23T13:49:11Z
2019-05-23T13:49:11Z
2013
Methods Mol Biol. 2013; 1000:91-101
10.1007/978-1-62703-405-0_8
1940-6029
1064-3745
Methods in molecular biology (Clifton, N.J.)
23585087
http://hdl.handle.net/20.500.12105/7671
In the search for novel biomarkers, noncandidate-based proteomic strategies open up new opportunities to gain a deeper insight into disease processes regarding their molecular mechanisms, the risk factors involved, and the monitoring of disease progression. To carry out these complex analyses, the combined use of gel electrophoresis with mass spectrometry (MS) represents a powerful choice. In addition, the introduction of protein dye labeling has notably improved the reliability of differential expression studies by increasing the statistical significance of the protein candidates. Here, we describe a strategy where different layers (luminal/abluminal) from the intraluminal thrombus (ILT) of human abdominal aortic aneurysm (AAA) patients were incubated in protein-free medium. Then, the levels of the proteins released were compared by two-dimensional differential in-gel electrophoresis (2D-DIGE) and the proteins of interest identified by MS. We consider that the use of tissue-conditioned media could offer a substantial advantage in the analytical study of biological fluids, as they provide a source of proteins to be released to the bloodstream, which could serve as potential circulating biomarkers.
eng
Identification of novel biomarkers of abdominal aortic aneurysms by 2D-DIGE and MALDI-MS from AAA-thrombus-conditioned media
journal article
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URL
https://repisalud.isciii.es/bitstreams/5f5405c1-ba62-4637-94ee-75381a1a7766/download
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application/pdf
IdentificationNovelBiomarkersAbdominal_2013.pdf
URL
https://repisalud.isciii.es/bitstreams/d5ed4469-7171-47ef-85de-8d6603f39faa/download
File
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IdentificationNovelBiomarkersAbdominal_2013.pdf.txt