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oai:repisalud.isciii.es:20.500.12105/76712024-09-27T10:13:24Zcom_20.500.12105_19604com_20.500.12105_2051col_20.500.12105_19607

00925njm 22002777a 4500 dc Martinez-Pinna, Roxana author Lopez, Juan Antonio author Ramos-Mozo, Priscila author Blanco-Colio, Luis M author Camafeita, Emilio author Calvo, Enrique author Meilhac, Olivier author Michel, Jean Baptiste author Egido, Jesús author Martin-Ventura, José Luis author 2013 In the search for novel biomarkers, noncandidate-based proteomic strategies open up new opportunities to gain a deeper insight into disease processes regarding their molecular mechanisms, the risk factors involved, and the monitoring of disease progression. To carry out these complex analyses, the combined use of gel electrophoresis with mass spectrometry (MS) represents a powerful choice. In addition, the introduction of protein dye labeling has notably improved the reliability of differential expression studies by increasing the statistical significance of the protein candidates. Here, we describe a strategy where different layers (luminal/abluminal) from the intraluminal thrombus (ILT) of human abdominal aortic aneurysm (AAA) patients were incubated in protein-free medium. Then, the levels of the proteins released were compared by two-dimensional differential in-gel electrophoresis (2D-DIGE) and the proteins of interest identified by MS. We consider that the use of tissue-conditioned media could offer a substantial advantage in the analytical study of biological fluids, as they provide a source of proteins to be released to the bloodstream, which could serve as potential circulating biomarkers. Methods Mol Biol. 2013; 1000:91-101 10.1007/978-1-62703-405-0_8 1940-6029 1064-3745 Methods in molecular biology (Clifton, N.J.) 23585087 http://hdl.handle.net/20.500.12105/7671 Identification of novel biomarkers of abdominal aortic aneurysms by 2D-DIGE and MALDI-MS from AAA-thrombus-conditioned media