2026-06-14T05:05:16Zhttps://repisalud.isciii.es/rest/oai/requestoai:repisalud.isciii.es:20.500.12105/76682024-09-27T10:06:48Zcom_20.500.12105_19604com_20.500.12105_2051col_20.500.12105_19607
Repisalud
author
Ramos-Mozo, Priscila
author
Madrigal-Matute, Julio
author
Martinez-Pinna, Roxana
author
Blanco-Colio, Luis Miguel
author
Lopez, Juan Antonio
author
Camafeita, Emilio
author
Meilhac, Olivier
author
Michel, Jean-Baptiste
author
Aparicio, Cesar
author
Vega de Ceniga, Melina
author
Egido, Jesus
author
Martín-Ventura, José Luis
funder
Unión Europea. Comisión Europea
funder
Ministerio de Ciencia e Innovación (España)
funder
Fundación Ramón Areces
funder
Ministerio de Sanidad y Consumo (España)
funder
Instituto de Salud Carlos III
funder
Fundación ProCNIC
2019-05-23T13:09:49Z
2019-05-23T13:09:49Z
2011-12
Arterioscler Thromb Vasc Biol. 2011; 31(12):3011-9
10.1161/ATVBAHA.111.237537
1524-4636
1079-5642
Arteriosclerosis, thrombosis, and vascular biology
21940941
http://hdl.handle.net/20.500.12105/7668
OBJECTIVE: Polymorphonuclear neutrophils (PMNs) play a main role in abdominal aortic aneurysm (AAA) progression. We have analyzed circulating PMNs isolated from AAA patients and controls by a proteomic approach to identify proteins potentially involved in AAA pathogenesis. METHODS AND RESULTS: PMNs from 8 AAA patients (4 large AAA >5 cm and 4 small AAA 3-5 cm) and 4 controls were analyzed by 2D differential in-gel electrophoresis. Among differentially expressed spots, several proteins involved in redox balance were identified by mass spectrometry (eg, cyclophilin, thioredoxin reductase, catalase). Diminished catalase expression and activity were observed in PMNs from AAA patients compared with controls. In contrast, PMNs from AAA patients displayed higher H(2)O(2) and myeloperoxidase levels than PMNs from controls. Moreover, a significant decrease in catalase mRNA levels was observed in PMNs after phorbol 12-myristate 13-acetate incubation. Catalase plasma levels were also decreased in large (n=47) and small (n=56) AAA patients compared with controls (n=34). We observed catalase expression in AAA thrombus and thrombus-conditioned medium, associated with PMN infiltration. Furthermore, increased H(2)O(2) levels were observed in AAA thrombus-conditioned medium compared with the media layer. CONCLUSIONS: Diminished catalase levels in circulating PMNs and plasma are observed in AAA patients, supporting an important role of oxidative stress in AAA evolution.
eng
Proteomic analysis of polymorphonuclear neutrophils identifies catalase as a novel biomarker of abdominal aortic aneurysm: potential implication of oxidative stress in abdominal aortic aneurysm progression
journal article
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ProteomicAnalysisPolymorphonuclearNeutrophils_2011.pdf
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text/plain
ProteomicAnalysisPolymorphonuclearNeutrophils_2011.pdf.txt