2026-06-14T12:13:08Zhttps://repisalud.isciii.es/rest/oai/requestoai:repisalud.isciii.es:20.500.12105/76672024-09-27T10:11:06Zcom_20.500.12105_19604com_20.500.12105_2051col_20.500.12105_19607
Repisalud
author
Rodriguez-Mahillo, Ana Isabel
author
Gonzalez-Muñoz, Miguel
author
Vega, José María
author
Lopez, Juan Antonio
author
Yart, Annie
author
Kerdelhué, Carole
author
Camafeita, Emilio
author
Garcia Ortiz, José Carlos
author
Vogel, Heiko
author
Petrucco Toffolo, Edoardo
author
Zovi, Daniel
author
Battisti, Andrea
author
Roques, Alain
author
Moneo, Ignacio
funder
Agence Nationale de la Recherche (Francia)
funder
Instituto de Salud Carlos III
funder
Ministerio de Ciencia e Innovación (España)
2019-05-23T12:32:56Z
2019-05-23T12:32:56Z
2012-12
Contact Dermatitis. 2012; 67(6):367-74
10.1111/j.1600-0536.2012.02107.x
1600-0536
01051873
Contact dermatitis
22708991
http://hdl.handle.net/20.500.12105/7667
BACKGROUND: Pine processionary larvae produce urticating hairs (setae) that serve for protection against predators. Setae induce cutaneous reactions in animals and humans. The presence of toxic or allergic mechanisms is a matter of debate. OBJECTIVES: To detect the presence of allergens in setae and to characterize them. MATERIALS AND METHODS: Setae extracts were characterized by gel staining and immunoblot, with sera from patients with immediate reactions and positive prick test reactions, as well as a rabbit antiserum raised against setae. Setae proteins were fractionated by high-performance liquid chromatography. The most relevant allergen was analysed by matrix-assisted laser desorption/ionization (MALDI) mass spectrometry (MS), and its sequence was deduced from an expressed sequence tag bank. Results. Setae contained at least seven different allergens. The most intense detection corresponded to a protein of MW ~ 14,000 that was similar to thaumetopoein, a previously described protein with mast cell-degranulating properties. MALDI-MS-based de novo sequencing provided a partial amino acid sequence different from that of the previously described allergen Tha p 1, and it was named Tha p 2. This allergen was detected in 61% of patients, and it is therefore a new major caterpillar allergen. CONCLUSIONS: Penetration of the setae from the pine processionary caterpillar delivers their allergenic content in addition to causing mechanical or toxic injury.
eng
Setae from the pine processionary moth (Thaumetopoea pityocampa) contain several relevant allergens
journal article
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URL
https://repisalud.isciii.es/bitstreams/0be89c48-c843-4ace-b3f5-f288ad7d0754/download
File
MD5
086e8824a171be59833288fa26d20d09
305907
application/pdf
SetaeFromThePineProcessionary_2012.pdf
URL
https://repisalud.isciii.es/bitstreams/bbc8f7c5-b8f4-42e8-8795-4f8252f276a5/download
File
MD5
456e8c27d5967f6d77598102f905513a
36105
text/plain
SetaeFromThePineProcessionary_2012.pdf.txt