2026-07-21T15:10:47Zhttps://repisalud.isciii.es/rest/oai/requestoai:repisalud.isciii.es:20.500.12105/76442025-06-13T09:06:32Zcom_20.500.12105_2052com_20.500.12105_2051col_20.500.12105_19617
Repisalud
author
Guijarro, Trinidad
author
Magro-Lopez, Esmeralda
author
Manso, Joana
author
Garcia-Martinez, Ricardo
author
Fernández-Aceñero, María Jesús
author
Liste-Noya, Isabel
author
Zambrano, Alberto
funder
Ministerio de Economía y Competitividad (España)
funder
Instituto de Salud Carlos III
2019-05-21T12:21:12Z
2019-05-21T12:21:12Z
2018
Mol Med. 2018 Dec 19;24(1):64.
10.1186/s10020-018-0064-z
1528-3658
1076-1551
Molecular medicine (Cambridge, Mass.)
30567504
http://hdl.handle.net/20.500.12105/7644
BACKGROUND: The multiple biological effects of vitamin D and its novel activities on inflammation and redox homeostasis have raised high expectations on its use as a therapeutic agent for multiple fibrogenic conditions. We have assessed the therapeutic effects of 1α,25-Dihydroxyvitamin D3, the biologically active form of vitamin D, in the context of lung fibrosis. METHODS: We have used representative cellular models for alveolar type II cells and human myofibroblasts. The extension of DNA damage and cellular senescence have been assessed by immunofluorescence, western-blot and senescence-associated β-galactosidase activity. We have also set up a murine model for lung fibrosis by intraperitoneal injections of bleomycin. RESULTS: Vitamin D induces cellular senescence in bleomycin-treated alveolar epithelial type II cells and aggravates the lung pathology induced by bleomycin. These effects are probably due to an alteration of the cellular DNA double-strand breaks repair in bleomycin-treated cells. CONCLUSIONS: The detrimental effects of vitamin D in the presence of a DNA damaging agent might preclude its use as an antifibrogenic agent for pulmonary fibrosis characterized by DNA damage occurrence and cellular senescence.
eng
Detrimental pro-senescence effects of vitamin D on lung fibrosis
research article
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URL
https://repisalud.isciii.es/bitstreams/f0050056-4153-45d8-b9f4-825a57bc3349/download
File
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DetrimentalProSenescenceEffects_2018.pdf
URL
https://repisalud.isciii.es/bitstreams/4ad0b895-9662-4608-8b30-083c708f5ed1/download
File
MD5
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DetrimentalProSenescenceEffects_2018.pdf.txt