2026-06-14T03:18:12Zhttps://repisalud.isciii.es/rest/oai/requestoai:repisalud.isciii.es:20.500.12105/76152024-09-27T23:34:47Zcom_20.500.12105_2052com_20.500.12105_2051col_20.500.12105_19609
Repisalud
author
Álvarez-Alonso, Raquel
author
Barandika, Jesús Félix
author
Ruiz-Fons, Francisco
author
Ortega-Araiztegi, Ione
author
Jado, Isabel
author
Hurtado, Ana
author
García-Pérez, Ana Luisa
funder
Instituto Nacional de Investigación y Tecnología Agraria y Alimentaria (España)
funder
Basque Government (España)
funder
Ministerio de Economía y Competitividad (España)
2019-05-21T07:52:47Z
2019-05-21T07:52:47Z
2018-11-20
Acta Vet Scand. 2018 Nov 20;60(1):75.
10.1186/s13028-018-0429-x
1751-0147
1751-0147
Acta veterinaria Scandinavica
30454011
http://hdl.handle.net/20.500.12105/7615
Bulk tank milk (BTM) samples were collected from 81 sheep flocks in the Basque Country, Spain, in 2015 and were analysed for antibodies against Coxiella burnetii by ELISA and for C. burnetii DNA by real-time PCR. Thirty-two percent of the flocks had BTM antibodies against C. burnetii. Presence of C. burnetii DNA in BTM was detected in 23% of the flocks, suggesting recent C. burnetii infections. Retrospective data of BTM samples obtained from 154 sheep flocks investigated in 2005 in the same geographic area were compiled to assess temporal changes in C. burnetii infection. The overall percentage of infected sheep flocks did not significantly change after the 10-year period. Among the 46 flocks sampled in both periods, 11 flocks that were negative in 2005 were positive in 2015, 18 maintained their initial status (positive or negative), and 17 positive flocks were negative in 2015. These findings indicate that C. burnetii infection is a dynamic process in dairy sheep in northern Spain. Single nucleotide polymorphism (SNP) genotyping of positive samples identified three genotypes, SNP1 being the most prevalent in 2015 and SNP8 in 2005; SNP4 was only detected once in 2005. These results suggest possible changes in the pattern of genotype infection over time.
eng
Bulk tank milk
Coxiella burnetii
Dairy sheep
SNP genotyping
Stable levels of Coxiella burnetii prevalence in dairy sheep flocks but changes in genotype distribution after a 10-year period in northern Spain
journal article
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URL
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application/pdf
StableLevelsOfCoxiella_2018.pdf
URL
https://repisalud.isciii.es/bitstreams/d1902f21-7648-40d6-a003-b2738b250b15/download
File
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text/plain
StableLevelsOfCoxiella_2018.pdf.txt