2026-06-14T03:31:25Zhttps://repisalud.isciii.es/rest/oai/request

oai:repisalud.isciii.es:20.500.12105/75842024-11-29T18:33:48Zcom_20.500.12105_2173com_20.500.12105_2051col_20.500.12105_19597

00925njm 22002777a 4500 dc Ferreirós, Alba author Pedrosa, Pablo author Da Silva-Álvarez, Sabela author Triana-Martínez, Francisco author Vilas, Jéssica M author Picallos-Rabina, Pilar author Gonzalez, Patricia author Gomez Gil, Maria author Li, Han author García-Caballero, Tomás author González-Barcia, Miguel author Vidal, Anxo author Collado, Manuel author 2019-05-14 Induction of pluripotency in somatic cells with defined genetic factors has been successfully used to investigate the mechanisms of disease initiation and progression. Cellular reprogramming and oncogenic transformation share common features; both involve undergoing a dramatic change in cell identity, and immortalization is a key step for cancer progression that enhances reprogramming. However, there are very few examples of complete successful reprogramming of tumor cells. Here we address the effect of expressing an active oncogene, RAS, on the process of reprogramming and found that, while combined expression with reprogramming factors enhanced dedifferentiation, expression within the context of neoplastic transformation impaired reprogramming. RAS induces expression changes that promote loss of cell identity and acquisition of stemness in a paracrine manner and these changes result in reprogramming when combined with reprogramming factors. When cells carry cooperating oncogenic defects, RAS drives cells into an incompatible cellular fate of malignancy. Stem Cell Reports. 2019;12(5):1099-1112. 10.1016/j.stemcr.2019.04.006 2213-6711 22136711 Stem cell reports 31056476 http://hdl.handle.net/20.500.12105/7584 Ras Cell plasticity Cell reprogramming iPSC Oncogenes Context-Dependent Impact of RAS Oncogene Expression on Cellular Reprogramming to Pluripotency