2026-08-29T19:46:52Zhttps://repisalud.isciii.es/rest/oai/request

oai:repisalud.isciii.es:20.500.12105/75792024-09-27T08:25:31Zcom_20.500.12105_19604com_20.500.12105_2051col_20.500.12105_19605

00925njm 22002777a 4500 dc Menchero, Sergio author Rollan, Isabel author Lopez-Izquierdo, Antonio author Andreu, Maria Jose author Sainz de Aja, Julio author Kang, Minjung author Adan, Javier author Benedito, Rui author Rayon, Teresa author Hadjantonakis, Anna-Katerina author Manzanares, Miguel author 2019-04-08 The Notch signalling pathway plays fundamental roles in diverse developmental processes in metazoans, where it is important in driving cell fate and directing differentiation of various cell types. However, we still have limited knowledge about the role of Notch in early preimplantation stages of mammalian development, or how it interacts with other signalling pathways active at these stages such as Hippo. By using genetic and pharmacological tools in vivo, together with image analysis of single embryos and pluripotent cell culture, we have found that Notch is active from the 4-cell stage. Transcriptomic analysis in single morula identified novel Notch targets, such as early naïve pluripotency markers or transcriptional repressors such as TLE4. Our results reveal a previously undescribed role for Notch in driving transitions during the gradual loss of potency that takes place in the early mouse embryo prior to the first lineage decisions. Elife. 2019; 8:e42930 10.7554/eLife.42930 2050-084X 2050-084X eLife 30958266 http://hdl.handle.net/20.500.12105/7579 Cdx2 Chromosomes Developmental biology Gene expression Morula Mouse Notch Preimplantation development Trophectoderm Transitions in cell potency during early mouse development are driven by Notch