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oai:repisalud.isciii.es:20.500.12105/75572024-09-27T08:07:00Zcom_20.500.12105_19604com_20.500.12105_2051col_20.500.12105_19605

00925njm 22002777a 4500 dc Evrard, Maximilien author Kwok, Immanuel W H author Chong, Shu Zhen author Teng, Karen W W author Becht, Etienne author Chen, Jinmiao author Sieow, Je Lin author Penny, Hweixian Leong author Ching, Goh Chi author Devi, Sapna author Adrover, Jose M author Li, Jackson L Y author Liong, Ka Hang author Tan, Leonard author Poon, Zhiyong author Foo, Shihui author Chua, Jia Wang author Su, I-Hsin author Balabanian, Karl author Bachelerie, Françoise author Biswas, Subhra K author Larbi, Anis author Hwang, William Y K author Madan, Vikas author Koeffler, H Phillip author Wong, Siew Cheng author Newell, Evan W author Hidalgo, Andres author Ginhoux, Florent author Ng, Lai Guan author 2018-02 Neutrophils are specialized innate cells that require constant replenishment from proliferative bone marrow (BM) precursors as a result of their short half-life. Although it is established that neutrophils are derived from the granulocyte-macrophage progenitor (GMP), the differentiation pathways from GMP to functional mature neutrophils are poorly defined. Using mass cytometry (CyTOF) and cell-cycle-based analysis, we identified three neutrophil subsets within the BM: a committed proliferative neutrophil precursor (preNeu) which differentiates into non-proliferating immature neutrophils and mature neutrophils. Transcriptomic profiling and functional analysis revealed that preNeu require the C/EBPε transcription factor for their generation from the GMP, and their proliferative program is substituted by a gain of migratory and effector function as they mature. preNeus expand under microbial and tumoral stress, and immature neutrophils are recruited to the periphery of tumor-bearing mice. In summary, our study identifies specialized BM granulocytic populations that ensure supply under homeostasis and stress responses. Immunity. 2018; 48(2):364-379 10.1016/j.immuni.2018.02.002 1097-4180 10747613 Immunity 29466759 http://hdl.handle.net/20.500.12105/7557 Granulopoiesis Neutrophil development Neutrophil ontogeny Neutrophil precursors Trafficking Developmental Analysis of Bone Marrow Neutrophils Reveals Populations Specialized in Expansion, Trafficking, and Effector Functions