2026-04-28T22:57:03Zhttps://repisalud.isciii.es/rest/oai/requestoai:repisalud.isciii.es:20.500.12105/75532024-09-27T10:07:14Zcom_20.500.12105_19604com_20.500.12105_2051col_20.500.12105_19607
Repisalud
author
Pérez-Pérez, Rafael
author
García-Santos, Eva
author
Ortega-Delgado, Francisco J
author
Lopez, Juan Antonio
author
Camafeita, Emilio
author
Ricart, Wifredo
author
Fernández-Real, José-Manuel
author
Peral, Belén
funder
Ministerio de Ciencia e Innovación (España)
funder
Instituto de Salud Carlos III
funder
Fundación ProCNIC
funder
Fundación Mutua Madrileña
2019-05-08T12:47:29Z
2019-05-08T12:47:29Z
2012-01-04
J Proteomics. 2012; 75(3):783-95
18743919
http://hdl.handle.net/20.500.12105/7553
21989264
10.1016/j.jprot.2011.09.016
1876-7737
Journal of proteomics
Obesity is recognized as an epidemic health problem worldwide. In humans, the accumulation of omental rather than subcutaneous fat appears to be tightly linked to insulin resistance, type 2 diabetes and cardiovascular disease. Differences in gene expression profiles in the adipose tissue comparing non-obese and obese subjects have been well documented. However, to date, no comparative proteomic studies based on omental fat have investigated the influence of obesity in protein expression. In this work, we searched for proteins differentially expressed in the omental fat of non-obese and obese subjects using 2D-DIGE and MS. Forty-four proteins, several of which were further studied by immunoblotting and immunostaining analyses, showed significant differences in the expression levels in the two groups of subjects. Our findings reveal a clearly distinctive proteomic profile between obese and non-obese subjects which emphasizes: i) reduced metabolic activity in the obese fat, since most down-regulated proteins were engaged in metabolic pathways; and ii) morphological and structural cell changes in the obese fat, as revealed by the functions exerted by most up-regulated proteins. Interestingly, transketolase and aminoacylase-1 represent newly described molecules involved in the pathophysiology of obesity, thus opening up new possibilities in the study of obesity.
eng
Attenuated metabolism is a hallmark of obesity as revealed by comparative proteomic analysis of human omental adipose tissue
journal article
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URL
https://repisalud.isciii.es/bitstreams/dfdb8a57-bc4f-49dd-af3c-82d33f75051d/download
File
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application/pdf
AttenuatedMetabolismHallmarkObesity_2012.pdf
URL
https://repisalud.isciii.es/bitstreams/41c4d343-dc18-4d8c-a08c-13bed3c878b1/download
File
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text/plain
AttenuatedMetabolismHallmarkObesity_2012.pdf.txt