2026-06-14T06:44:17Zhttps://repisalud.isciii.es/rest/oai/requestoai:repisalud.isciii.es:20.500.12105/75482024-09-27T08:37:52Zcom_20.500.12105_19604com_20.500.12105_2051col_20.500.12105_19605col_20.500.12105_19607
Repisalud
author
Villarroya-Beltri, Carolina
author
Gutierrez-Vazquez, Cristina
author
Sanchez-Cabo, Fatima
author
Perez-Hernandez, Daniel
author
Vazquez, Jesus
author
Martin-Cofreces, Noa B.
author
Martinez-Herrera, Dannys Jorge
author
Pascual-Montano, Alberto
author
Mittelbrunn, Maria
author
Sanchez-Madrid, Francisco
funder
Unión Europea. Comisión Europea
funder
Unión Europea. Comisión Europea. European Research Council (ERC)
funder
Instituto de Salud Carlos III
funder
Ministerio de Educación (España)
funder
Comunidad de Madrid (España)
funder
Fundación La Caixa
2019-05-07T11:37:49Z
2019-05-07T11:37:49Z
2013
Nat Commun. 2013; 4:2980
10.1038/ncomms3980
2041-1723
2041-1723
Nature communications
24356509
http://hdl.handle.net/20.500.12105/7548
Exosomes are released by most cells to the extracellular environment and are involved in cell-to-cell communication. Exosomes contain specific repertoires of mRNAs, microRNAs (miRNAs) and other non-coding RNAs that can be functionally transferred to recipient cells. However, the mechanisms that control the specific loading of RNA species into exosomes remain unknown. Here we describe sequence motifs present in miRNAs that control their localization into exosomes. The protein heterogeneous nuclear ribonucleoprotein A2B1 (hnRNPA2B1) specifically binds exosomal miRNAs through the recognition of these motifs and controls their loading into exosomes. Moreover, hnRNPA2B1 in exosomes is sumoylated, and sumoylation controls the binding of hnRNPA2B1 to miRNAs. The loading of miRNAs into exosomes can be modulated by mutagenesis of the identified motifs or changes in hnRNPA2B1 expression levels. These findings identify hnRNPA2B1 as a key player in miRNA sorting into exosomes and provide potential tools for the packaging of selected regulatory RNAs into exosomes and their use in biomedical applications.
eng
Sumoylated hnRNPA2B1 controls the sorting of miRNAs into exosomes through binding to specific motifs
journal article
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URL
https://repisalud.isciii.es/bitstreams/8fa8c31c-9996-4741-bf90-5a8d85053d70/download
File
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application/pdf
SumoylatedHnRNPA2B1controls _2013.pdf
URL
https://repisalud.isciii.es/bitstreams/94ed9d0f-79e6-4658-b30c-231813581078/download
File
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text/plain
SumoylatedHnRNPA2B1controls _2013.pdf.txt