2026-06-14T02:28:54Zhttps://repisalud.isciii.es/rest/oai/request

oai:repisalud.isciii.es:20.500.12105/75092024-09-27T08:42:20Zcom_20.500.12105_19604com_20.500.12105_2051col_20.500.12105_19605

00925njm 22002777a 4500 dc Sainz de Aja, Julio author Menchero, Sergio author Rollan, Isabel author Barral, Antonio author Tiana, Maria author Jawaid, Wajid author Cossio, Itziar author Alvarez, Alba author Carreño-Tarragona, Gonzalo author Badia-Careaga, Claudio author Nichols, Jennifer author Göttgens, Berthold author Isern, Joan author Manzanares, Miguel author 2019-04-01 Progenitors of the first hematopoietic cells in the mouse arise in the early embryo from Brachyury-positive multipotent cells in the posterior-proximal region of the epiblast, but the mechanisms that specify primitive blood cells are still largely unknown. Pluripotency factors maintain uncommitted cells of the blastocyst and embryonic stem cells in the pluripotent state. However, little is known about the role played by these factors during later development, despite being expressed in the postimplantation epiblast. Using a dual transgene system for controlled expression at postimplantation stages, we found that Nanog blocks primitive hematopoiesis in the gastrulating embryo, resulting in a loss of red blood cells and downregulation of erythropoietic genes. Accordingly, Nanog-deficient embryonic stem cells are prone to erythropoietic differentiation. Moreover, Nanog expression in adults prevents the maturation of erythroid cells. By analysis of previous data for NANOG binding during stem cell differentiation and CRISPR/Cas9 genome editing, we found that Tal1 is a direct NANOG target. Our results show that Nanog regulates primitive hematopoiesis by directly repressing critical erythroid lineage specifiers. EMBO J. 2019; 38(7):e99122 10.15252/embj.201899122 1460-2075 0261-4189 The EMBO journal 30814124 http://hdl.handle.net/20.500.12105/7509 Nanog Tal1 Erythropoiesis Gastrulation Primitive hematopoiesis The pluripotency factor NANOG controls primitive hematopoiesis and directly regulates Tal1