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00925njm 22002777a 4500 dc Dominguez-Rodriguez, Mercedes author Toraño, Alfredo author 1999-01-04 To mimic the sandfly pool feeding process and characterize the cellular and biochemical events that occur during the early stages of promastigote-host interaction, we developed an ex vivo model of human blood infection with Leishmania promastigotes. Within 30 s of blood contact, Leishmania promastigotes bind natural anti-Leishmania antibodies, which then activate the classical complement pathway and opsonization by the third component of complement. The opsonized promastigotes undergo an immune adherence reaction and bind quantitatively to erythrocyte CR1 receptors; opsonized Leishmania amastigotes also bind to erythrocytes. Progression of infection implies promastigote transfer from erythrocytes to acceptor blood leukocytes. After 10 min of ex vivo infection, 25% of all leukocytes contain intracellular parasites, indicating that blood cells are the early targets for the invading promastigotes. We propose that adaptation to the immune adherence mechanism aids Leishmania survival, promoting rapid promastigote phagocytosis by leukocytes. This facilitates host colonization and may represent the parasite's earliest survival strategy. In light of this mechanism, it is unlikely that infection-blocking vaccines can be developed. J Exp Med. 1999;189(1):25-35. 0022-1007 The Journal of experimental medicine https://pmc.ncbi.nlm.nih.gov/articles/PMC1887685/ 9874561 http://hdl.handle.net/20.500.12105/7474 Leishmania Infection Immune adherence Natural antibodies Complement Immune adherence-mediated opsonophagocytosis: the mechanism of Leishmania infection