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oai:repisalud.isciii.es:20.500.12105/74302024-09-27T10:17:01Zcom_20.500.12105_2173com_20.500.12105_2051col_20.500.12105_19597

00925njm 22002777a 4500 dc Castaño, Julio author Bueno, Clara author Jiménez-Delgado, Senda author Roca-Ho, Heleia author Fraga, Mario F author Fernandez, Agustín F author Nakanishi, Mahito author Torres-Ruiz Raul, Raúl author Rodriguez Perales, Sandra author Menéndez, Pablo author 2017 We report the generation-characterization of a fetal liver (FL) B-cell progenitor (BCP)-derived human induced pluripotent stem cell (hiPSC) line CRISPR/Cas9-edited to carry/express a single copy of doxycycline-inducible Cas9 gene in the "safe locus" AAVS1 (iCas9-FL-BCP-hiPSC). Gene-edited iPSCs remained pluripotent after CRISPR/Cas9 genome-edition. Correct genomic integration of a unique copy of Cas9 was confirmed by PCR and Southern blot. Cas9 was robustly and specifically expressed on doxycycline exposure. T7-endonuclease assay demonstrated that iCas9 induces robust gene-edition when gRNAs against hematopoietic transcription factors were tested. This iCas9-FL-BCP-hiPSC will facilitate gene-editing approaches for studies on developmental biology, drug screening and disease modeling. Stem Cell Res. 2017;21:137-140 10.1016/j.scr.2017.04.011 1876-7753 18735061 Stem cell research 28677529 http://hdl.handle.net/20.500.12105/7430 Generation and characterization of a human iPSC cell line expressing inducible Cas9 in the "safe harbor" AAVS1 locus