2026-07-21T11:58:41Zhttps://repisalud.isciii.es/rest/oai/requestoai:repisalud.isciii.es:20.500.12105/74232024-09-27T23:48:23Zcom_20.500.12105_2052com_20.500.12105_2051col_20.500.12105_19609
Repisalud
author
Gago Prieto, Sara
author
Lorenzo, Belen
author
Gomez-Lopez, Alicia
author
Cuesta de la Plaza, Isabel
author
Cuenca-Estrella, Manuel
author
Buitrago, Maria Jose
funder
Instituto de Salud Carlos III
funder
Ministerio de Ciencia e Innovación (España)
2019-04-02T09:59:30Z
2019-04-02T09:59:30Z
2013-01-23
BMC Microbiol. 2013 Jan 23;13:13.
10.1186/1471-2180-13-13
1471-2180
BMC microbiology
23343107
http://hdl.handle.net/20.500.12105/7423
BACKGROUND: Several genotyping protocols have been described to study Candida albicans strains with different sensitivity values. In this study we have analyzed the genetic relatedness and the antifungal susceptibility of several Candida albicans strains isolated from a patient who from suffered recurrent candiduria for a period of five years. Strains were genotyped using Microsatellite Length Polymorphism (MLP) with three microsatellite markers (HIS 3, EF 3 and CDC 3), and a new method based on high resolution melting (HRM) was developed to analyze the microsatellite region. This method was compared with the conventional technique that uses capillary electrophoresis. RESULTS: MICs of the isolates showed the existence of fluconazole susceptible and resistant strains. An inter-colony test using single concentration (8 and 16 mg/l) of fluconazole revealed the coexistence of both fluconazole susceptible and resistant strains. Both genotyping analysis methods showed that all the patient's isolates had a clonal origin. HRM analysis method developed was able to accurately establish strain relatedness and presented a discriminatory power of 0.77. CONCLUSIONS: Although HRM analysis method presented a lower discriminatory power compared to methods based on capillary electrophoresis, it provided a more cost-effective and suitable alternative for genotyping C. albicans in a clinical laboratory.
eng
Analysis of strain relatedness using high resolution melting in a case of recurrent candiduria
journal article
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AnalysisOfStrainRelatedness_2013.pdf
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AnalysisOfStrainRelatedness_2013.pdf.txt