2026-06-14T00:28:29Zhttps://repisalud.isciii.es/rest/oai/requestoai:repisalud.isciii.es:20.500.12105/73772025-04-03T13:08:41Zcom_20.500.12105_2052com_20.500.12105_2051col_20.500.12105_19609
Repisalud
author
Flores-Chavez, Maria
author
Gonzalez, Luis Miguel
author
Hurtado, Carolina
author
Monje Motta, Yamileth
author
Dominguez-Hidalgo, Cristina
author
Merino, Francisco Jesús
author
Perteguer-Prieto, Maria Jesus
author
Garate, Teresa
funder
Instituto de Salud Carlos III
funder
Ministerio de Economía y Competitividad (España)
2019-03-26T13:47:23Z
2019-03-26T13:47:23Z
2018-01-29
Parasit Vectors. 2018 Feb 27;11(1):117.
10.1186/s13071-018-2646-6
1756-3305
Parasites & vectors
29486767
http://hdl.handle.net/20.500.12105/7377
BACKGROUND: Taenia solium, T. asiatica and T. saginata tapeworms cause human taeniasis and are the origin of porcine and bovine cysticercosis. Furthermore, T. solium eggs can cause human cysticercosis, with neurocysticercosis being the most serious form of the disease. These helminth infections are neglected tropical diseases and are endemic in several countries in the Americas, Asia and Africa. As a result of globalization, migration in particular, the infections have been extending to non-endemic territories. Species-specific diagnosis of taeniasis is subject to drawbacks that could be resolved using molecular approaches. In the present study, conventional and real-time amplification protocols (cPCR and qPCR) based on the T. saginata HDP2 sequence were applied in the differential diagnosis of taeniasis (T. saginata, T. solium) in both fecal samples and proglottids expelled by patients. The HDP2 homolog in T. solium was cloned and characterized. RESULTS: Semi-nested cPCR and qPCR (Sn-HDP2 cPCR and Sn-HDP2 qPCR) amplified T. saginata and T. solium DNA, with an analytical sensitivity of 40 and 400 fg, respectively, and identically in both protocols. Eighteen taeniasis patients were diagnosed directly with T. saginata or T. solium, either from proglottids or fecal samples with/without eggs (detected using microscopy), based on the optimized Sn-HDP2 qPCR. After cloning, the T. solium HDP2 homolog sequence was confirmed to be a ribosomal sequence. The HDP2 fragment corresponded to a non-transcribed sequence/external transcribed repeat (NTS/ETS) of ribosomal DNA. Compared with the T. saginata HDP2 homolog, the T solium HDP2 sequence lacked the first 900 nt at the 5' end and showed nucleotide substitutions and small deletions. CONCLUSIONS: Sn-HDP2 cPCR and Sn-HDP2 qPCR were set up for the diagnosis of human taeniasis, using proglottids and fecal samples from affected patients. The new Sn-HDP2 qPCR protocol was the best option, as it directly differentiated T. saginata from T. solium. The diagnosis of an imported T. solium-taeniasis case and nine European T. saginata cases was relevant. Finally, the cloning and sequencing of the T. solium HDP2 fragment confirmed that HDP2 was part of a ribosomal unit.
eng
Diagnosis
Ribosomal DNA
Taenia saginata
Taenia solium
Taeniasis
cPCR
qPCR
HDP2: a ribosomal DNA (NTS-ETS) sequence as a target for species-specific molecular diagnosis of intestinal taeniasis in humans
research article
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https://repisalud.isciii.es/bitstreams/0c47fa6d-2734-45c8-8501-868fcf425b5f/download
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application/pdf
HDP2_DNA_sequence.pdf
URL
https://repisalud.isciii.es/bitstreams/ce08d72d-8b93-4d6c-8ca7-318384e4ed0d/download
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2d8089f29fb2dc4b5112dc0275459784
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text/plain
HDP2_DNA_sequence.pdf.txt