2026-05-22T00:57:19Zhttps://repisalud.isciii.es/rest/oai/request

oai:repisalud.isciii.es:20.500.12105/73652025-05-05T11:30:12Zcom_20.500.12105_2052com_20.500.12105_2051col_20.500.12105_19609

00925njm 22002777a 4500 dc Otón, Joaquín author Pereiro, Eva author Conesa, José J author Chichón, Francisco Javier author Luque, Daniel author Rodriguez Martinez, Javier M author Pérez-Berná, Ana J author Sorzano, Carlos Oscar S author Klukowska, Joanna author Herman, Gabor T author Vargas, Javier author Marabini, Roberto author Carrascosa, José L author Carazo, José M author 2017-04-04 We have developed a new data collection method and processing framework in full field cryo soft X-ray tomography to computationally extend the depth of field (DOF) of a Fresnel zone plate lens. Structural features of 3D-reconstructed eukaryotic cells that are affected by DOF artifacts in standard reconstruction are now recovered. This approach, based on focal series projections, is easily applicable with closed expressions to select specific data acquisition parameters. Sci Rep. 2017 Apr;7:45808 10.1038/srep45808 2045-2322 Scientific Reports 28374769 http://hdl.handle.net/20.500.12105/7365 XTEND: Extending the depth of field in cryo soft X-ray tomography