2026-05-17T05:46:40Zhttps://repisalud.isciii.es/rest/oai/requestoai:repisalud.isciii.es:20.500.12105/73522024-09-27T10:14:56Zcom_20.500.12105_19604com_20.500.12105_2051col_20.500.12105_19607
Repisalud
author
Toran, Jose Luis
author
Lopez, Juan Antonio
author
Gomes-Alves, Patricia
author
Aguilar, Susana
author
Torroja, Carlos
author
Trevisan-Herraz, Marco
author
Moscoso, Isabel
author
Sebastião, Maria João
author
Serra, Margarida
author
Brito, Catarina
author
Cruz, Francisco Miguel
author
Sepulveda, Juan Carlos
author
Abad, José Luis
author
Galan-Arriola, Carlos
author
Ibáñez, Borja
author
Martinez, Fernando
author
Fernández, María Eugenia
author
Fernández-Avilés, Francisco
author
Palacios, Itziar
author
R-Borlado, Luis
author
Vazquez, Jesus
author
Alves, Paula M
author
Bernad, Antonio
funder
Unión Europea. Comisión Europea
funder
Ministerio de Ciencia e Innovación (España)
funder
Comunidad de Madrid (España)
funder
Instituto de Salud Carlos III
funder
Fundação para a Ciência e Tecnologia (Portugal)
funder
Ministério da Educação e Ciência (Portugal)
funder
Unión Europea. Fondo Europeo de Desarrollo Regional (FEDER/ERDF)
2019-03-18T13:23:41Z
2019-03-18T13:23:41Z
2019-03-15
Sci Rep. 2019; 9(1):4647
10.1038/s41598-019-39571-x
2045-2322
2045-2322
Scientific reports
30874584
http://hdl.handle.net/20.500.12105/7352
Adult cardiac progenitor/stem cells (CPC/CSC) are multipotent resident populations involved in cardiac homeostasis and heart repair. Assisted by complementary RNAseq analysis, we defined the fraction of the CPC proteome associable with specific functions by comparison with human bone marrow mesenchymal stem cells (MSC), the reference population for cell therapy, and human dermal fibroblasts (HDF), as a distant reference. Label-free proteomic analysis identified 526 proteins expressed differentially in CPC. iTRAQ analysis confirmed differential expression of a substantial proportion of those proteins in CPC relative to MSC, and systems biology analysis defined a clear overrepresentation of several categories related to enhanced angiogenic potential. The CPC plasma membrane compartment comprised 1,595 proteins, including a minimal signature of 167 proteins preferentially or exclusively expressed by CPC. CDH5 (VE-cadherin), OX2G (OX-2 membrane glycoprotein; CD200), GPR4 (G protein-coupled receptor 4), CACNG7 (calcium voltage-gated channel auxiliary subunit gamma 7) and F11R (F11 receptor; junctional adhesion molecule A; JAM-A; CD321) were selected for validation. Their differential expression was confirmed both in expanded CPC batches and in early stages of isolation, particularly when compared against cardiac fibroblasts. Among them, GPR4 demonstrated the highest discrimination capacity between all cell lineages analyzed.
eng
Definition of a cell surface signature for human cardiac progenitor cells after comprehensive comparative transcriptomic and proteomic characterization
journal article
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