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oai:repisalud.isciii.es:20.500.12105/73522024-09-27T10:14:56Zcom_20.500.12105_19604com_20.500.12105_2051col_20.500.12105_19607

00925njm 22002777a 4500 dc Toran, Jose Luis author Lopez, Juan Antonio author Gomes-Alves, Patricia author Aguilar, Susana author Torroja, Carlos author Trevisan-Herraz, Marco author Moscoso, Isabel author Sebastião, Maria João author Serra, Margarida author Brito, Catarina author Cruz, Francisco Miguel author Sepulveda, Juan Carlos author Abad, José Luis author Galan-Arriola, Carlos author Ibáñez, Borja author Martinez, Fernando author Fernández, María Eugenia author Fernández-Avilés, Francisco author Palacios, Itziar author R-Borlado, Luis author Vazquez, Jesus author Alves, Paula M author Bernad, Antonio author 2019-03-15 Adult cardiac progenitor/stem cells (CPC/CSC) are multipotent resident populations involved in cardiac homeostasis and heart repair. Assisted by complementary RNAseq analysis, we defined the fraction of the CPC proteome associable with specific functions by comparison with human bone marrow mesenchymal stem cells (MSC), the reference population for cell therapy, and human dermal fibroblasts (HDF), as a distant reference. Label-free proteomic analysis identified 526 proteins expressed differentially in CPC. iTRAQ analysis confirmed differential expression of a substantial proportion of those proteins in CPC relative to MSC, and systems biology analysis defined a clear overrepresentation of several categories related to enhanced angiogenic potential. The CPC plasma membrane compartment comprised 1,595 proteins, including a minimal signature of 167 proteins preferentially or exclusively expressed by CPC. CDH5 (VE-cadherin),  OX2G (OX-2 membrane glycoprotein; CD200), GPR4 (G protein-coupled receptor 4), CACNG7 (calcium voltage-gated channel auxiliary subunit gamma 7) and F11R (F11 receptor; junctional adhesion molecule A; JAM-A; CD321) were selected for validation. Their differential expression was confirmed both in expanded CPC batches and in early stages of isolation, particularly when compared against cardiac fibroblasts. Among them, GPR4 demonstrated the highest discrimination capacity between all cell lineages analyzed. Sci Rep. 2019; 9(1):4647 10.1038/s41598-019-39571-x 2045-2322 2045-2322 Scientific reports 30874584 http://hdl.handle.net/20.500.12105/7352 Definition of a cell surface signature for human cardiac progenitor cells after comprehensive comparative transcriptomic and proteomic characterization