2026-08-05T15:42:45Zhttps://repisalud.isciii.es/rest/oai/request

oai:repisalud.isciii.es:20.500.12105/72952024-09-27T09:02:23Zcom_20.500.12105_19604com_20.500.12105_2051col_20.500.12105_19605

00925njm 22002777a 4500 dc Rey, María-Dolores author Castillejo, María Ángeles author Sánchez-Lucas, Rosa author Guerrero-Sanchez, Victor M author López-Hidalgo, Cristina author Romero-Rodríguez, Cristina author Valero-Galván, José author Sghaier-Hammami, Besma author Simova-Stoilova, Lyudmila author Echevarría-Zomeño, Sira author Jorge, Inmaculada author Gómez-Gálvez, Isabel author Papa, María Eugenia author Carvalho, Kamilla author Rodríguez de Francisco, Luis E author Maldonado-Alconada, Ana María author Valledor, Luis author Jorrín-Novo, Jesús V author 2019-02-06 Proteomics has had a big impact on plant biology, considered as a valuable tool for several forest species, such as Quercus, Pines, Poplars, and Eucalyptus. This review assesses the potential and limitations of the proteomics approaches and is focused on Quercus ilex as a model species and other forest tree species. Proteomics has been used with Q. ilex since 2003 with the main aim of examining natural variability, developmental processes, and responses to biotic and abiotic stresses as in other species of the genus Quercus or Pinus. As with the progress in techniques in proteomics in other plant species, the research in Q. ilex moved from 2-DE based strategy to the latest gel-free shotgun workflows. Experimental design, protein extraction, mass spectrometric analysis, confidence levels of qualitative and quantitative proteomics data, and their interpretation are a true challenge with relation to forest tree species due to their extreme orphan and recalcitrant (non-orthodox) nature. Implementing a systems biology approach, it is time to validate proteomics data using complementary techniques and integrate it with the -omics and classical approaches. The full potential of the protein field in plant research is quite far from being entirely exploited. However, despite the methodological limitations present in proteomics, there is no doubt that this discipline has contributed to deeper knowledge of plant biology and, currently, is increasingly employed for translational purposes. Int J Mol Sci. 2019; 20(3):692 10.3390/ijms20030692 1422-0067 1422-0067 International journal of molecular sciences 30736277 http://hdl.handle.net/20.500.12105/7295 2-DE proteomics Quercus ilex holm oak Non-orthodox seed Population variability Shotgun proteomics Stresses responses Proteomics, Holm Oak (Quercus ilex L.) and Other Recalcitrant and Orphan Forest Tree Species: How do They See Each Other?