2026-06-14T05:06:57Zhttps://repisalud.isciii.es/rest/oai/requestoai:repisalud.isciii.es:20.500.12105/72782024-09-27T21:18:37Zcom_20.500.12105_2052com_20.500.12105_2051col_20.500.12105_19609
Repisalud
author
Trevijano-Contador, Nuria
author
Rossi, Suelen Andreia
author
Alves, Elisabete
author
Landín-Ferreiroa, Santiago
author
Zaragoza, Oscar
funder
Ministerio de Economía y Competitividad (España)
funder
Ciências sem fronteiras
2019-03-02T21:02:22Z
2019-03-02T21:02:22Z
2017-07-31
Front Microbiol. 2017;31;8:1423.
10.3389/fmicb.2017.01423
1664-302X
Frontiers in microbiology
28824559
http://hdl.handle.net/20.500.12105/7278
Cryptococcus neoformans is an environmental encapsulated yeast that behaves as an opportunistic pathogen in immunocompromised individuals. The capsule is the main virulence factor of this pathogen. This structure is highly dynamic, and it can change its size and structure according to the environmental conditions. During infection, C. neoformans significantly enlarges the size of the capsule by the addition of new polysaccharide. It is believed that capsule growth is an energy-cost process, but this aspect has never been addressed. In this work, we have evaluated the role of mitochondrial activity on capsule growth using specific inhibitors of the electron respiratory chain. We observed that capsule growth was impaired in the presence of inhibitors of the respiratory chain as salicylhydroxamic acid or antimycin A. Furthermore, capsule growth correlated with an increase of the mitochondrial membrane potential and higher production of reactive oxygen species. Our results confirm that capsule growth depends on mitochondrial activity, and open new insights to understand the regulation of this process.
eng
Cryptococcus neoformans
Capsule enlargement
Mitochondria
Mitochondrial membrane potential
Reactive oxygen species
Capsule Enlargement in Cryptococcus neoformans Is Dependent on Mitochondrial Activity
journal article
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CapsuleEnlargementInCryptococcus_2017.pdf
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CapsuleEnlargementInCryptococcus_2017.pdf.txt