2026-06-30T03:03:20Zhttps://repisalud.isciii.es/rest/oai/requestoai:repisalud.isciii.es:20.500.12105/72552025-05-09T09:01:06Zcom_20.500.12105_2052com_20.500.12105_2051col_20.500.12105_19609
Repisalud
author
Méndez, Jairo A
author
Usme-Ciro, Jose A
author
Domingo Carrasco, Cristina
author
Rey, Gloria J
author
Sánchez, Juan A
author
Tenorio, Antonio
author
Gallego-Gomez, Juan C
funder
Gobierno de Colombia
funder
Instituto Colombiano para el Desarrollo de la Ciencia y la Tecnología Francisco José de Caldas (Colombia)
funder
Instituto Nacional de Salud (Colombia)
2019-02-28T12:15:58Z
2019-02-28T12:15:58Z
2012-03-09
Virol J. 2012 Mar 9;9:64.
10.1186/1743-422X-9-64
1743-422X
Virology journal
22405440
http://hdl.handle.net/20.500.12105/7255
BACKGROUND: Dengue fever is perhaps the most important viral re-emergent disease especially in tropical and sub-tropical countries, affecting about 50 million people around the world yearly. In Colombia, dengue virus was first detected in 1971 and still remains as a major public health issue. Although four viral serotypes have been recurrently identified, dengue virus type 2 (DENV-2) has been involved in the most important outbreaks during the last 20 years, including 2010 when the fatality rate highly increased. As there are no major studies reviewing virus origin and genotype distribution in this country, the present study attempts to reconstruct the phylogenetic history of DENV-2 using a sequence analysis from a 224 bp PCR-amplified product corresponding to the carboxyl terminus of the envelope (E) gene from 48 Colombian isolates. RESULTS: As expected, the oldest isolates belonged to the American genotype (subtype V), but the strains collected since 1990 represent the American/Asian genotype (subtype IIIb) as previously reported in different American countries. Interestingly, the introduction of this genotype coincides with the first report of dengue hemorrhagic fever in Colombia at the end of 1989 and the increase of cases during the next years. CONCLUSION: After replacement of the American genotype, several lineages of American/Asian subtype have rapidly spread all over the country evolving in new clades. Nevertheless, the direct association of these new variants in the raise of lethality rate observed during the last outbreak has to be demonstrated.
eng
Phylogenetic reconstruction of dengue virus type 2 in Colombia
research article
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URL
https://repisalud.isciii.es/bitstreams/97111849-81ce-4b11-bdf4-41c3ba0d8d98/download
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PhylogeneticReconstructionOfDengue_2012.pdf
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https://repisalud.isciii.es/bitstreams/0ac6f5cd-41dd-4f5d-8675-83034ac0cc9a/download
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PhylogeneticReconstructionOfDengue_2012.pdf.txt