2026-07-21T17:02:07Zhttps://repisalud.isciii.es/rest/oai/requestoai:repisalud.isciii.es:20.500.12105/72512025-06-06T08:51:09Zcom_20.500.12105_2052com_20.500.12105_2051col_20.500.12105_19609
Repisalud
author
Nieto, Amelia
author
Pozo Sanchez, Francisco
author
Vidal-García, Matxalen
author
Omeñaca, Manuel
author
Casas Flecha, Inmaculada
author
Falcon, Ana
funder
Ministerio de Economía, Industria y Competitividad (España)
funder
Instituto de Salud Carlos III
2019-02-27T19:35:30Z
2019-02-27T19:35:30Z
2017-04
Front Microbiol. 2017 Apr 3;8:575.
10.3389/fmicb.2017.00575
1664-302X
Frontiers in microbiology
28421062
http://hdl.handle.net/20.500.12105/7251
Several amino acid changes have been previously implicated in adaptation of avian influenza viruses to human hosts, among them the D701N change in the PB2 polymerase subunit that also is the main determinant of avian virus pathogenesis in animal models. However, previous studies using recombinant viruses did not provide conclusive information of the contribution of this PB2 residue to pathogenicity in human influenza virus strains. We identified this mutation in an A(H1N1)pdm09-like human influenza virus isolated from an infected patient with pneumonia and acute respiratory failure, admitted to the intensive care unit. An exhaustive search has revealed PB2-D701 as a highly conserved position in all available H1N1 human virus sequences in NCBI database, showing a very low prevalence of PB2-D701N change. Presence of PB2-701N amino acid correlates with severe or fatal outcome in those scarce cases with known disease outcome of the infection. In these patients, the residue PB2-701N may contribute to pathogenicity as it was previously reported in humans infected with avian viruses. This study helps to clarify a debate that has arisen regarding the role of PB2-D701N in human influenza virus pathogenicity.
eng
D701N mutation
PB2 subunit
Adaptation of avian viruses
Influenza virus
Pathogenicity
Identification of Rare PB2-D701N Mutation from a Patient with Severe Influenza: Contribution of the PB2-D701N Mutation to the Pathogenicity of Human Influenza
research article
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IdentificationOfRarePB2_2017.pdf
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CorrigendumIdentificationOfRare_2017.pdf
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IdentificationOfRarePB2_2017.pdf.txt
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CorrigendumIdentificationOfRare_2017.pdf.txt