2026-08-05T15:04:32Zhttps://repisalud.isciii.es/rest/oai/requestoai:repisalud.isciii.es:20.500.12105/72452025-03-27T10:05:59Zcom_20.500.12105_2052com_20.500.12105_2051col_20.500.12105_19609
Repisalud
author
Putri, Rindia M.
author
Allende-Ballestero, Carolina
author
Luque, Daniel
author
Klem, Robin
author
Rousou, Katerina-Asteria
author
Liu, Aijie
author
Traulsen, Christoph H.-H.
author
Rurup, W. Frederik
author
Koay, Melissa S. T.
author
Castón, José R
author
Cornelissen, Jeroen J. L. M.
funder
Unión Europea. Comisión Europea. European Research Council (ERC)
funder
Indonesia Endowment Fund for Education (LPDP)
funder
Ministerio de Economía y Competitividad (España)
funder
Comunidad de Madrid (España)
2019-02-27T18:26:16Z
2019-02-27T18:26:16Z
2017-11-22
ACS Nano. 2017;26;11(12):12796-12804.
10.1021/acsnano.7b07669
1936-086X
1936-0851
ACS Nano
29166561
http://hdl.handle.net/20.500.12105/7245
Recent years have witnessed the emergence of bacterial semiorganelle encapsulins as promising platforms for bio-nanotechnology. To advance the development of encapsulins as nanoplatforms, a functional and structural basis of these assemblies is required. Encapsulin from Brevibacterium linens is known to be a protein-based vessel for an enzyme cargo in its cavity, which could be replaced with a foreign cargo, resulting in a modified encapsulin. Here, we characterize the native structure of B. linens encapsulins with both native and foreign cargo using cryo-electron microscopy (cryo-EM). Furthermore, by harnessing the confined enzyme (i.e., a peroxidase), we demonstrate the functionality of the encapsulin for an in vitro surface-immobilized catalysis in a cascade pathway with an additional enzyme, glucose oxidase. We also demonstrate the in vivo functionality of the encapsulin for cellular uptake using mammalian macrophages. Unraveling both the structure and functionality of the encapsulins allows transforming biological nanocompartments into functional systems.
eng
Bacterial compartments
Cryo-electron microscopy
Encapsulin
Nanoplatforms
Nanoreactors
Structural Characterization of Native and Modified Encapsulins as Nanoplatforms for in Vitro Catalysis and Cellular Uptake
research article
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URL
https://repisalud.isciii.es/bitstreams/e1534f7e-1bbb-406e-b1cd-047c461ba296/download
File
MD5
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application/pdf
PMC6150732_StructuralCharacterizationOfNative_2017.pdf
URL
https://repisalud.isciii.es/bitstreams/43e9541c-3150-4c18-95c3-1fb3c0ad9568/download
File
MD5
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text/plain
PMC6150732_StructuralCharacterizationOfNative_2017.pdf.txt