2026-08-29T13:36:38Zhttps://repisalud.isciii.es/rest/oai/request

oai:repisalud.isciii.es:20.500.12105/72402025-05-07T10:14:30Zcom_20.500.12105_2173com_20.500.12105_2051col_20.500.12105_19597

00925njm 22002777a 4500 dc Ecker, Simone author Chen, Lu author Pancaldi, Vera author Bagger, Frederik O author Fernández, José María author Carrillo-de-Santa-Pau, Enrique author Juan, David author Mann, Alice L author Watt, Stephen author Casale, Francesco Paolo author Sidiropoulos, Nikos author Rapin, Nicolas author Merkel, Angelika author Stunnenberg, Hendrik G author Stegle, Oliver author Frontini, Mattia author Downes, Kate author Pastinen, Tomi author Kuijpers, Taco W author Rico, Daniel author Valencia, Alfonso author Beck, Stephan author Soranzo, Nicole author Paul, Dirk S author 2017-01-26 BACKGROUND: A healthy immune system requires immune cells that adapt rapidly to environmental challenges. This phenotypic plasticity can be mediated by transcriptional and epigenetic variability. RESULTS: We apply a novel analytical approach to measure and compare transcriptional and epigenetic variability genome-wide across CD14+CD16- monocytes, CD66b+CD16+ neutrophils, and CD4+CD45RA+ naïve T cells from the same 125 healthy individuals. We discover substantially increased variability in neutrophils compared to monocytes and T cells. In neutrophils, genes with hypervariable expression are found to be implicated in key immune pathways and are associated with cellular properties and environmental exposure. We also observe increased sex-specific gene expression differences in neutrophils. Neutrophil-specific DNA methylation hypervariable sites are enriched at dynamic chromatin regions and active enhancers. CONCLUSIONS: Our data highlight the importance of transcriptional and epigenetic variability for the key role of neutrophils as the first responders to inflammatory stimuli. We provide a resource to enable further functional studies into the plasticity of immune cells, which can be accessed from: http://blueprint-dev.bioinfo.cnio.es/WP10/hypervariability . Genome Biol. 2017;18(1):18. 10.1186/s13059-017-1156-8 1474-760X 1474-760X Genome biology 28126036 http://hdl.handle.net/20.500.12105/7240 DNA methylation Differential variability Gene expression Heterogeneity Immune cells Monocytes Neutrophils Phenotypic plasticity T cells Genome-wide analysis of differential transcriptional and epigenetic variability across human immune cell types