2026-06-14T05:05:29Zhttps://repisalud.isciii.es/rest/oai/requestoai:repisalud.isciii.es:20.500.12105/72392024-11-29T16:18:04Zcom_20.500.12105_2173com_20.500.12105_2051col_20.500.12105_19597
Repisalud
author
Gómez-Cabello, Daniel
author
Checa-Rodríguez, Cintia
author
Abad, María
author
Serrano Marugan, Manuel
author
Huertas, Pablo
funder
Ministerio de Ciencia y Competitividad (España)
funder
Unión Europea. Comisión Europea. European Research Council (ERC)
2019-02-27T09:46:19Z
2019-02-27T09:46:19Z
2017-02-14
Stem Cell Reports. 2017;8(2):432-445.
10.1016/j.stemcr.2016.12.009
2213-6711
22136711
Stem cell reports
28065643
http://hdl.handle.net/20.500.12105/7239
Acquired genomic instability is one of the major concerns for the clinical use of induced pluripotent stem cells (iPSCs). All reprogramming methods are accompanied by the induction of DNA damage, of which double-strand breaks are the most cytotoxic and mutagenic. Consequently, DNA repair genes seem to be relevant for accurate reprogramming to minimize the impact of such DNA damage. Here, we reveal that reprogramming is associated with high levels of DNA end resection, a critical step in homologous recombination. Moreover, the resection factor CtIP is essential for cell reprogramming and establishment of iPSCs, probably to repair reprogramming-induced DNA damage. Our data reveal a new role for DNA end resection in maintaining genomic stability during cell reprogramming, allowing DNA repair fidelity to be retained in both human and mouse iPSCs. Moreover, we demonstrate that reprogramming in a resection-defective environment has long-term consequences on stem cell self-renewal and differentiation.
eng
DNA resection
Cell reprogramming
Genetic instability
iPSC
CtIP-Specific Roles during Cell Reprogramming Have Long-Term Consequences in the Survival and Fitness of Induced Pluripotent Stem Cells
journal article
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CtIP-SpecificRolesduring_2017.pdf
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CtIP-SpecificRolesduring-_2017-mmc1.pdf
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CtIP-SpecificRolesduring_2017.pdf.txt
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CtIP-SpecificRolesduring-_2017-mmc1.pdf.txt
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