2026-06-14T03:30:06Zhttps://repisalud.isciii.es/rest/oai/request

oai:repisalud.isciii.es:20.500.12105/72382025-04-29T10:18:59Zcom_20.500.12105_2173com_20.500.12105_2051col_20.500.12105_19597

00925njm 22002777a 4500 dc Torres-Ruiz Raul, Raul author Martinez-Lage, Marta author Martin, Maria C author Garcia, Aida author Bueno, Clara author Castaño, Julio author Ramirez, Juan C author Menéndez, Pablo author Cigudosa, Juan C author Rodriguez Perales, Sandra author 2017 Efficient methodologies for recreating cancer-associated chromosome translocations are in high demand as tools for investigating how such events initiate cancer. The CRISPR/Cas9 system has been used to reconstruct the genetics of these complex rearrangements at native loci while maintaining the architecture and regulatory elements. However, the CRISPR system remains inefficient in human stem cells. Here, we compared three strategies aimed at enhancing the efficiency of the CRISPR-mediated t(11;22) translocation in human stem cells, including mesenchymal and induced pluripotent stem cells: (1) using end-joining DNA processing factors involved in repair mechanisms, or (2) ssODNs to guide the ligation of the double-strand break ends generated by CRISPR/Cas9; and (3) all-in-one plasmid or ribonucleoprotein complex-based approaches. We report that the generation of targeted t(11;22) is significantly increased by using a combination of ribonucleoprotein complexes and ssODNs. The CRISPR/Cas9-mediated generation of targeted t(11;22) in human stem cells opens up new avenues in modeling Ewing sarcoma. Stem Cell Reports. 2017;8(5):1408-1420. 10.1016/j.stemcr.2017.04.014 2213-6711 22136711 Stem cell reports 28494941 http://hdl.handle.net/20.500.12105/7238 CRISPR Cas9 Ewing sarcoma MSC Cancer modeling Cancer translocation Disease model Genome engineering Human stem cells iPSC Efficient Recreation of t(11;22) EWSR1-FLI1+ in Human Stem Cells Using CRISPR/Cas9