2026-07-21T11:35:04Zhttps://repisalud.isciii.es/rest/oai/request

oai:repisalud.isciii.es:20.500.12105/71842024-09-27T20:37:46Zcom_20.500.12105_2052com_20.500.12105_2051col_20.500.12105_19609

00925njm 22002777a 4500 dc González-de la Fuente, Sandra author Peiró-Pastor, Ramón author Rastrojo, Alberto author Moreno, Javier author Carrasco-Ramiro, Fernando author Requena, Jose M author Aguado, Begoña author 2017-12-22 Leishmania parasites are the causative of leishmaniasis, a group of potentially fatal human diseases. Control strategies for leishmaniasis can be enhanced by genome based investigations. The publication in 2005 of the Leishmania major genome sequence, and two years later the genomes for the species Leishmania braziliensis and Leishmania infantum were major milestones. Since then, the L. infantum genome, although highly fragmented and incomplete, has been used widely as the reference genome to address whole transcriptomics and proteomics studies. Here, we report the sequencing of the L. infantum genome by two NGS methodologies and, as a result, the complete genome assembly on 36 contigs (chromosomes). Regarding the present L. infantum genome-draft, 495 new genes have been annotated, a hundred have been corrected and 75 previous annotated genes have been discontinued. These changes are not only the result of an increase in the genome size, but a significant contribution derives from the existence of a large number of incorrectly assembled regions in current chromosomal scaffolds. Furthermore, an improved assembly of tandemly repeated genes has been obtained. All these analyses support that the de novo assembled L. infantum genome represents a robust assembly and should replace the currently available in the databases. Sci Rep. 2017 Dec 22;7(1):18050. 10.1038/s41598-017-18374-y 2045-2322 Scientific reports 29273719 http://hdl.handle.net/20.500.12105/7184 Resequencing of the Leishmania infantum (strain JPCM5) genome and de novo assembly into 36 contigs