2026-06-14T03:39:42Zhttps://repisalud.isciii.es/rest/oai/requestoai:repisalud.isciii.es:20.500.12105/71792025-05-30T16:29:05Zcom_20.500.12105_2052com_20.500.12105_2051col_20.500.12105_19609
Repisalud
author
Ramírez, Laura
author
Iborra, Salvador
author
Cortés, Jimena
author
Bonay, Pedro
author
Alonso, Carlos
author
Barral-Netto, Manoel
author
Soto, Manuel
funder
Ministerio de Ciencia e Innovación (España)
funder
Instituto de Salud Carlos III
funder
Agencia Española de Cooperación Internacional para el Desarrollo
funder
Programa Iberoamericano de Ciencia y Tecnología para el Desarrollo (España)
funder
Laboratorios LETI
2019-02-19T09:52:57Z
2019-02-19T09:52:57Z
2010-06
J Biomed Biotechnol. 2010;2010:181690.
10.1155/2010/181690
1110-7251
1110-7243
Journal of Biomedicine and Biotechnology
20145701
http://hdl.handle.net/20.500.12105/7179
Leishmaniasis is an increasing public health problem and effective vaccines are not currently available. We have previously demonstrated that vaccination with ribosomal proteins extracts administered in combination of CpG oligodeoxynucleotides protects susceptible BALB/c mice against primary Leishmania major infection. Here, we evaluate the long-term immunity to secondary infection conferred by this vaccine. We show that vaccinated and infected BALB/c mice were able to control a secondary Leishmania major challenge, since no inflammation and very low number of parasites were observed in the site of reinfection. In addition, although an increment in the parasite burden was observed in the draining lymph nodes of the primary site of infection we did not detected inflammatory lesions at that site. Resistance against reinfection correlated to a predominant Th1 response against parasite antigens. Thus, cell cultures established from spleens and the draining lymph node of the secondary site of infection produced high levels of parasite specific IFN-gamma in the absence of IL-4 and IL-10 cytokine production. In addition, reinfected mice showed a high IgG2a/IgG1 ratio for anti-Leishmania antibodies. Our results suggest that ribosomal vaccine, which prevents pathology in a primary challenge, in combination with parasite persistence might be effective for long-term maintenance of immunity.
eng
BALB/c Mice Vaccinated withLeishmania majorRibosomal Proteins Extracts Combined with CpG Oligodeoxynucleotides Become Resistant to Disease Caused by a Secondary Parasite Challenge
research article
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URL
https://repisalud.isciii.es/bitstreams/f513c05b-2405-441f-b2a8-680acb27f114/download
File
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application/pdf
BALBcMiceVaccinatedWith2010.pdf
URL
https://repisalud.isciii.es/bitstreams/33d2962c-303b-48bb-8d52-9181d37a22a0/download
File
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text/plain
BALBcMiceVaccinatedWith2010.pdf.txt