2026-06-14T00:29:36Zhttps://repisalud.isciii.es/rest/oai/requestoai:repisalud.isciii.es:20.500.12105/71772025-06-12T13:38:40Zcom_20.500.12105_2052com_20.500.12105_2051col_20.500.12105_19609
Repisalud
author
Gonçalves, Samuel M
author
Lagrou, Katrien
author
Rodrigues, Cláudia S
author
Campos, Cláudia F
author
Bernal-Martinez, Leticia
author
Rodrigues, Fernando
author
Silvestre, Ricardo
author
Alcazar-Fuoli, Laura
author
Maertens, Johan A
author
Cunha, Cristina
author
Carvalho, Agostinho
funder
Institut Mérieux
funder
Unión Europea. Fondo Europeo de Desarrollo Regional (FEDER/ERDF)
funder
Fundação para a Ciência e Tecnologia (Portugal)
funder
Instituto de Salud Carlos III
2019-02-18T12:43:31Z
2019-02-18T12:43:31Z
2017-11-29
Front Microbiol. 2017 Nov 29;8:2362.
10.3389/fmicb.2017.02362
1664-302X
Frontiers in microbiology
29238334
http://hdl.handle.net/20.500.12105/7177
Background: Invasive pulmonary aspergillosis (IPA) is an infection that primarily affects immunocompromised hosts, including hematological patients and stem-cell transplant recipients. The diagnosis of IPA remains challenging, making desirable the availability of new specific biomarkers. High-throughput methods now allow us to interrogate the immune system for multiple markers of inflammation with enhanced resolution. Methods: To determine whether a signature of alveolar cytokines could be associated with the development of IPA and used as a diagnostic biomarker, we performed a nested case-control study involving 113 patients at-risk. Results: Among the 32 analytes tested, IL-1β, IL-6, IL-8, IL-17A, IL-23, and TNFα were significantly increased among patients with IPA, defining two clusters able to accurately differentiate cases of infection from controls. Genetic variants previously reported to confer increased risk of IPA compromised the production of specific cytokines and impaired their discriminatory potential toward infection. Collectively, our data indicated that IL-8 was the best performing cytokine, with alveolar levels ≥904 pg/mL predicting IPA with elevated sensitivity (90%), specificity (73%), and negative predictive value (88%). Conclusions: These findings highlight the existence of a specific profile of alveolar cytokines, with IL-8 being the dominant discriminator, which might be useful in supporting current diagnostic approaches for IPA.
eng
Biomarkers
Bronchoalveolar lavage
Cytokines/chemokines
Fungal diagnostics
Invasive pulmonary aspergillosis
Evaluation of Bronchoalveolar Lavage Fluid Cytokines as Biomarkers for Invasive Pulmonary Aspergillosis in At-Risk Patients
research article
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EvaluationOfBronchoalveolarLavage_2017.pdf
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EvaluationOfBronchoalveolarLavage_2017.pdf.txt