2026-06-14T03:33:18Zhttps://repisalud.isciii.es/rest/oai/request

oai:repisalud.isciii.es:20.500.12105/71772025-06-12T13:38:40Zcom_20.500.12105_2052com_20.500.12105_2051col_20.500.12105_19609

00925njm 22002777a 4500 dc Gonçalves, Samuel M author Lagrou, Katrien author Rodrigues, Cláudia S author Campos, Cláudia F author Bernal-Martinez, Leticia author Rodrigues, Fernando author Silvestre, Ricardo author Alcazar-Fuoli, Laura author Maertens, Johan A author Cunha, Cristina author Carvalho, Agostinho author 2017-11-29 Background: Invasive pulmonary aspergillosis (IPA) is an infection that primarily affects immunocompromised hosts, including hematological patients and stem-cell transplant recipients. The diagnosis of IPA remains challenging, making desirable the availability of new specific biomarkers. High-throughput methods now allow us to interrogate the immune system for multiple markers of inflammation with enhanced resolution. Methods: To determine whether a signature of alveolar cytokines could be associated with the development of IPA and used as a diagnostic biomarker, we performed a nested case-control study involving 113 patients at-risk. Results: Among the 32 analytes tested, IL-1β, IL-6, IL-8, IL-17A, IL-23, and TNFα were significantly increased among patients with IPA, defining two clusters able to accurately differentiate cases of infection from controls. Genetic variants previously reported to confer increased risk of IPA compromised the production of specific cytokines and impaired their discriminatory potential toward infection. Collectively, our data indicated that IL-8 was the best performing cytokine, with alveolar levels ≥904 pg/mL predicting IPA with elevated sensitivity (90%), specificity (73%), and negative predictive value (88%). Conclusions: These findings highlight the existence of a specific profile of alveolar cytokines, with IL-8 being the dominant discriminator, which might be useful in supporting current diagnostic approaches for IPA. Front Microbiol. 2017 Nov 29;8:2362. 10.3389/fmicb.2017.02362 1664-302X Frontiers in microbiology 29238334 http://hdl.handle.net/20.500.12105/7177 Biomarkers Bronchoalveolar lavage Cytokines/chemokines Fungal diagnostics Invasive pulmonary aspergillosis Evaluation of Bronchoalveolar Lavage Fluid Cytokines as Biomarkers for Invasive Pulmonary Aspergillosis in At-Risk Patients