2026-06-14T03:29:54Zhttps://repisalud.isciii.es/rest/oai/requestoai:repisalud.isciii.es:20.500.12105/71742025-06-17T07:01:38Zcom_20.500.12105_2052com_20.500.12105_2051col_20.500.12105_19609
Repisalud
author
Seiringer, Peter
author
Pritsch, Michael
author
Flores-Chavez, Maria
author
Marchisio, Edoardo
author
Helfrich, Kerstin
author
Mengele, Carolin
author
Hohnerlein, Stefan
author
Bretzel, Gisela
author
Löscher, Thomas
author
Hoelscher, Michael
author
Berens-Riha, Nicole
funder
German Center for Infection Research (Alemania)
funder
Ludwig-Maximilians-Universität München (Alemania)
2019-02-13T12:35:21Z
2019-02-13T12:35:21Z
2017-04-07
Diagn Microbiol Infect Dis. 2017;88(3):225-232
10.1016/j.diagmicrobio.2017.04.003
1879-0070
07328893
Diagnostic Microbiology and Infectious Disease
28456430
http://hdl.handle.net/20.500.12105/7174
Due to increased migration, Chagas disease has become an international health problem. Reliable diagnosis of chronically infected people is crucial for prevention of non-vectorial transmission as well as treatment. This study compared four distinct PCR methods for detection of Trypanosoma cruzi DNA for the use in well-equipped routine diagnostic laboratories. DNA was extracted of T. cruzi-positive and negative patients' blood samples and cultured T. cruzi, T. rangeli as well as Leishmania spp. One conventional and two real-time PCR methods targeting a repetitive Sat-DNA sequence as well as one conventional PCR method targeting the variable region of the kDNA minicircle were compared for sensitivity, intra- and interassay precision, limit of detection, specificity and cross-reactivity. Considering the performance, costs and ease of use, an algorithm for PCR-diagnosis of patients with a positive serology for T. cruzi antibodies was developed.
eng
Comparison
Conventional
Diagnosis
PCR
Real-time
Trypanosoma cruzi
Comparison of four PCR methods for efficient detection of Trypanosoma cruzi in routine diagnostics
research article
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URL
https://repisalud.isciii.es/bitstreams/46fdbb11-e53a-4250-8dfa-0c2eacc32687/download
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application/pdf
ComparisonOfFourPCR_2017.pdf
URL
https://repisalud.isciii.es/bitstreams/18538693-cd5f-43fb-bf1b-7419a08b9a1a/download
File
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text/plain
ComparisonOfFourPCR_2017.pdf.txt