2026-06-14T03:32:22Zhttps://repisalud.isciii.es/rest/oai/request

oai:repisalud.isciii.es:20.500.12105/71742025-06-17T07:01:38Zcom_20.500.12105_2052com_20.500.12105_2051col_20.500.12105_19609

00925njm 22002777a 4500 dc Seiringer, Peter author Pritsch, Michael author Flores-Chavez, Maria author Marchisio, Edoardo author Helfrich, Kerstin author Mengele, Carolin author Hohnerlein, Stefan author Bretzel, Gisela author Löscher, Thomas author Hoelscher, Michael author Berens-Riha, Nicole author 2017-04-07 Due to increased migration, Chagas disease has become an international health problem. Reliable diagnosis of chronically infected people is crucial for prevention of non-vectorial transmission as well as treatment. This study compared four distinct PCR methods for detection of Trypanosoma cruzi DNA for the use in well-equipped routine diagnostic laboratories. DNA was extracted of T. cruzi-positive and negative patients' blood samples and cultured T. cruzi, T. rangeli as well as Leishmania spp. One conventional and two real-time PCR methods targeting a repetitive Sat-DNA sequence as well as one conventional PCR method targeting the variable region of the kDNA minicircle were compared for sensitivity, intra- and interassay precision, limit of detection, specificity and cross-reactivity. Considering the performance, costs and ease of use, an algorithm for PCR-diagnosis of patients with a positive serology for T. cruzi antibodies was developed. Diagn Microbiol Infect Dis. 2017;88(3):225-232 10.1016/j.diagmicrobio.2017.04.003 1879-0070 07328893 Diagnostic Microbiology and Infectious Disease 28456430 http://hdl.handle.net/20.500.12105/7174 Comparison Conventional Diagnosis PCR Real-time Trypanosoma cruzi Comparison of four PCR methods for efficient detection of Trypanosoma cruzi in routine diagnostics