2026-06-14T05:06:54Zhttps://repisalud.isciii.es/rest/oai/request

oai:repisalud.isciii.es:20.500.12105/71472024-09-27T09:24:36Zcom_20.500.12105_19604com_20.500.12105_2051col_20.500.12105_19605

00925njm 22002777a 4500 dc Ivanovitch, Kenzo author Termino, Susana author Torres, Miguel author 2017 During vertebrate heart development, two progenitor populations, first and second heart fields (FHF, SHF), sequentially contribute to longitudinal subdivisions of the heart tube (HT), with the FHF contributing the left ventricle and part of the atria, and the SHF the rest of the heart. Here, we study the dynamics of cardiac differentiation and morphogenesis by tracking individual cells in live analysis of mouse embryos. We report that during an initial phase, FHF precursors differentiate rapidly to form a cardiac crescent, while limited morphogenesis takes place. In a second phase, no differentiation occurs while extensive morphogenesis, including splanchnic mesoderm sliding over the endoderm, results in HT formation. In a third phase, cardiac precursor differentiation resumes and contributes to SHF-derived regions and the dorsal closure of the HT. These results reveal tissue-level coordination between morphogenesis and differentiation during HT formation and provide a new framework to understand heart development. Elife. 2017; 6:e30668 10.7554/eLife.30668 2050-084X 2050-084X eLife 29202929 http://hdl.handle.net/20.500.12105/7147 Cardiac crescent Cardiac development Developmental biology First heart field Heart tube Live-imaging Mouse Second heart field Stem cells Live imaging of heart tube development in mouse reveals alternating phases of cardiac differentiation and morphogenesis