2026-06-14T06:44:39Zhttps://repisalud.isciii.es/rest/oai/requestoai:repisalud.isciii.es:20.500.12105/71292024-10-31T11:39:45Zcom_20.500.12105_19604com_20.500.12105_2051col_20.500.12105_19605
Repisalud
author
Le Garrec, Jean-François
author
Domínguez, Jorge N
author
Desgrange, Audrey
author
Ivanovitch, Kenzo
author
Raphaël, Etienne
author
Bangham, J Andrew
author
Torres, Miguel
author
Coen, Enrico
author
Mohun, Timothy J
author
Meilhac, Sigolène M
funder
Agence Nationale de la Recherche (Francia)
funder
Institut National de la Santé et de la Recherche Médicale (Francia)
funder
Institut Pasteur
funder
Ministerio de Economía, Industria y Competitividad (España)
2019-02-06T09:08:13Z
2019-02-06T09:08:13Z
2017
Elife. 2017; 6:e28951
10.7554/eLife.28951
2050-084X
2050-084X
eLife
29179813
http://hdl.handle.net/20.500.12105/7129
How left-right patterning drives asymmetric morphogenesis is unclear. Here, we have quantified shape changes during mouse heart looping, from 3D reconstructions by HREM. In combination with cell labelling and computer simulations, we propose a novel model of heart looping. Buckling, when the cardiac tube grows between fixed poles, is modulated by the progressive breakdown of the dorsal mesocardium. We have identified sequential left-right asymmetries at the poles, which bias the buckling in opposite directions, thus leading to a helical shape. Our predictive model is useful to explore the parameter space generating shape variations. The role of the dorsal mesocardium was validated in Shh-/- mutants, which recapitulate heart shape changes expected from a persistent dorsal mesocardium. Our computer and quantitative tools provide novel insight into the mechanism of heart looping and the contribution of different factors, beyond the simple description of looping direction. This is relevant to congenital heart defects.
eng
3D shape
Computer modelling
Developmental biology
Heart morphogenesis
Left-right patterning
Mouse
Stem cells
A predictive model of asymmetric morphogenesis from 3D reconstructions of mouse heart looping dynamics
journal article
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