2026-06-14T03:31:41Zhttps://repisalud.isciii.es/rest/oai/request

oai:repisalud.isciii.es:20.500.12105/71292024-10-31T11:39:45Zcom_20.500.12105_19604com_20.500.12105_2051col_20.500.12105_19605

00925njm 22002777a 4500 dc Le Garrec, Jean-François author Domínguez, Jorge N author Desgrange, Audrey author Ivanovitch, Kenzo author Raphaël, Etienne author Bangham, J Andrew author Torres, Miguel author Coen, Enrico author Mohun, Timothy J author Meilhac, Sigolène M author 2017 How left-right patterning drives asymmetric morphogenesis is unclear. Here, we have quantified shape changes during mouse heart looping, from 3D reconstructions by HREM. In combination with cell labelling and computer simulations, we propose a novel model of heart looping. Buckling, when the cardiac tube grows between fixed poles, is modulated by the progressive breakdown of the dorsal mesocardium. We have identified sequential left-right asymmetries at the poles, which bias the buckling in opposite directions, thus leading to a helical shape. Our predictive model is useful to explore the parameter space generating shape variations. The role of the dorsal mesocardium was validated in Shh-/- mutants, which recapitulate heart shape changes expected from a persistent dorsal mesocardium. Our computer and quantitative tools provide novel insight into the mechanism of heart looping and the contribution of different factors, beyond the simple description of looping direction. This is relevant to congenital heart defects. Elife. 2017; 6:e28951 10.7554/eLife.28951 2050-084X 2050-084X eLife 29179813 http://hdl.handle.net/20.500.12105/7129 3D shape Computer modelling Developmental biology Heart morphogenesis Left-right patterning Mouse Stem cells A predictive model of asymmetric morphogenesis from 3D reconstructions of mouse heart looping dynamics