2026-06-14T03:32:18Zhttps://repisalud.isciii.es/rest/oai/requestoai:repisalud.isciii.es:20.500.12105/70672024-09-27T22:21:26Zcom_20.500.12105_2052com_20.500.12105_2051col_20.500.12105_19609
Repisalud
author
Hyams, Catherine
author
Opel, Sophia
author
Hanage, William
author
Yuste, Jose Enrique
author
Bax, Katie
author
Henriques-Normark, Birgitta
author
Spratt, Brian G
author
Brown, Jeremy S
funder
National Institute for Health Research (Reino Unido)
2019-02-01T15:41:14Z
2019-02-01T15:41:14Z
2011-10
PLoS One. 2011;6(10):e24581.
10.1371/journal.pone.0024581
1932-6203
1932-6203
PloS one
22022358
http://hdl.handle.net/20.500.12105/7067
BACKGROUND: Immunity to infections caused by Streptococcus pneumoniae is dependent on complement. There are wide variations in sensitivity to complement between S. pneumoniae strains that could affect their ability to cause invasive infections. Although capsular serotype is one important factor causing differences in complement resistance between strains, there is also considerable other genetic variation between S. pneumoniae strains that may affect complement-mediated immunity. We have therefore investigated whether genetically distinct S. pneumoniae strains with the same capsular serotype vary in their sensitivity to complement mediated immunity. METHODOLOGY AND PRINCIPAL FINDINGS: C3b/iC3b deposition and neutrophil association were measured using flow cytometry assays for S. pneumoniae strains with different genetic backgrounds for each of eight capsular serotypes. For some capsular serotypes there was marked variation in C3b/iC3b deposition between different strains that was independent of capsule thickness and correlated closely to susceptibility to neutrophil association. C3b/iC3b deposition results also correlated weakly with the degree of IgG binding to each strain. However, the binding of C1q (the first component of the classical pathway) correlated more closely with C3b/iC3b deposition, and large differences remained in complement sensitivity between strains with the same capsular serotype in sera in which IgG had been cleaved with IdeS. CONCLUSIONS: These data demonstrate that bacterial factors independent of the capsule and recognition by IgG have strong effects on the susceptibility of S. pneumoniae to complement, and could therefore potentially account for some of the differences in virulence between strains.
eng
Effects of Streptococcus pneumoniae strain background on complement resistance
journal article
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URL
https://repisalud.isciii.es/bitstreams/187c7d71-a522-4686-8390-863340496421/download
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application/pdf
EffectsOfStreptococcusPneumoniae_2011.pdf
URL
https://repisalud.isciii.es/bitstreams/e430e903-571a-4eb4-920f-45800130ef0a/download
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EffectsOfStreptococcusPneumoniae_2011.pdf.txt