2026-06-14T03:30:39Zhttps://repisalud.isciii.es/rest/oai/requestoai:repisalud.isciii.es:20.500.12105/70642024-09-27T10:09:11Zcom_20.500.12105_2052com_20.500.12105_2051com_20.500.12105_19604col_20.500.12105_19609col_20.500.12105_19607
Repisalud
author
Bhide, Mangesh Ramesh
author
Escudero, Raquel
author
Camafeita, Emilio
author
Gil, Horacio
author
Jado, Isabel
author
Anda, Pedro
funder
Ministerio de Educación y Ciencia (España)
funder
Instituto de Salud Carlos III
2019-02-01T14:34:07Z
2019-02-01T14:34:07Z
2009-07-15
BMC Res Notes. 2009 Jul 15;2:134.
10.1186/1756-0500-2-134
1756-0500
BMC research notes
19604355
http://hdl.handle.net/20.500.12105/7064
BACKGROUND: Borreliae employ multiple immune evasive strategies such as binding to complement regulatory proteins [factor H (fH) and factor H like-1 (FHL1)], differential regulation of surface membrane proteins, antigenic variation, and binding of plasminogen/plasmin and matrix metalloproteinases. As a complement regulatory subunit, fH serves as a cofactor for the factor I-mediated cleavage of C3b. fH binding by Borrelia has been correlated with pathogenesis as well as with host diversity. Here we show the differential binding of borrelial proteins to fH from human and animal sera. FINDINGS: Affinity ligand binding experiments, 2-D electrophoresis, and protein identification and peptide de novo sequencing based on mass spectrometry, revealed novel fH putative binding proteins of Lyme- and relapsing fever Borrelia. An OspA serotype-associated differential human and animal fH binding by B. garinii was also observed, which could be related with the ability of some strains from serotypes 4 and 7 to invade non-nervous system tissues. Also, the variable affinity of binding proteins expressed by different Borrelia to animal fH correlated with their host selectivity. CONCLUSION: The novel animal and human putative fH binding proteins (FHBPs) in this study underscore the importance of evasion of complement in the pathogenesis of Borrelia infections.
eng
Complement factor H binding by different Lyme disease and relapsing fever Borrelia in animals and human
research article
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URL
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File
MD5
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ComplementFactorHBinding_2009.pdf
URL
https://repisalud.isciii.es/bitstreams/9f9e2105-928d-40e4-9519-5a49e396a3b7/download
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text/plain
ComplementFactorHBinding_2009.pdf.txt