2026-07-21T15:38:55Zhttps://repisalud.isciii.es/rest/oai/requestoai:repisalud.isciii.es:20.500.12105/70382025-06-30T11:04:41Zcom_20.500.12105_2052com_20.500.12105_2051com_20.500.12105_15322col_20.500.12105_19608col_20.500.12105_16961col_20.500.12105_16962col_20.500.12105_16964col_20.500.12105_16970col_20.500.12105_16983
Repisalud
author
Casabonne, Delphine
author
Benavente, Yolanda
author
Robles, Claudia
author
Costas, Laura
author
Alonso, Esther
author
Gonzalez-Barca, Eva
author
Tardón, Adonina
author
Dierssen-Sotos, Trinidad
author
Vázquez, Eva Gimeno
author
Aymerich, Marta
author
Campo, Elias
author
Castaño-Vinyals, Gemma
author
Aragones, Nuria
author
Pollan-Santamaria, Marina
author
Kogevinas, Manolis
author
Juwana, Hedy
author
Middeldorp, Jaap
author
de Sanjose, Silvia
funder
Instituto de Investigación Marqués de Valdecilla
funder
Fundación Cajastur
funder
Asociación Española Contra el Cáncer
funder
Instituto de Salud Carlos III
funder
Fundación La Caixa
funder
Government of Catalonia (España)
2019-01-31T09:57:50Z
2019-01-31T09:57:50Z
2015-02-09
Infect Agent Cancer. 2015 Feb 9;10:5.
10.1186/1750-9378-10-5
1750-9378
Infectious agents and cancer
25972916
http://hdl.handle.net/20.500.12105/7038
BACKGROUND: Epstein-Barr virus (EBV)-related malignancies harbour distinct serological responses to EBV antigens. We hypothesized that EBV serological patterns can be useful to identify different stages of chronic lymphocytic leukemia. METHODS: Information on 150 cases with chronic lymphocytic leukemia and 157 frequency-matched (by age, sex and region) population-based controls from a Spanish multicentre case-control study was obtained. EBV immunoglobulin G serostatus was evaluated through a peptide-based ELISA and further by immunoblot analysis to EBV early antigens (EA), nuclear antigen (EBNA1), VCA-p18, VCA-p40 and Zebra. Two independent individuals categorized the serological patterns of the western blot analysis. Patients with very high response and diversity in EBV-specific polypeptides, in particular with clear responses to EA-associated proteins, were categorized as having an abnormal reactive pattern (ab_EBV). Adjusted odds ratios (OR) and 95% confidence interval (CI) were estimated using logistic regression models. RESULTS: Almost all subjects were EBV-IgG positive (>95% of cases and controls) whereas ab_EBV patterns were detected in 23% of cases (N = 34) and 11% of controls (N = 17; OR: 2.44, 95% CI, 1.29 to 4.62; P = 0.006), particularly in intermediate/high risk patients. Although based on small numbers, the association was modified by smoking with a gradual reduction of ab_EBV-related OR for all Rai stages from never smokers to current smokers. CONCLUSIONS: Highly distinct EBV antibody diversity patterns revealed by immunoblot analysis were detected in cases compared to controls, detectable at very early stages of the disease and particularly among non smokers. This study provides further evidence of an abnormal immunological response against EBV in patients with chronic lymphocytic leukemia.
eng
Case-control
Chronic lymphocytic leukemia
Epstein-Barr virus
Serology
Smoking
Aberrant Epstein-Barr virus antibody patterns and chronic lymphocytic leukemia in a Spanish multicentric case-control study
research article
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AberrantEpsteinBarrVirus_2015.pdf.txt