2026-06-14T03:33:15Zhttps://repisalud.isciii.es/rest/oai/requestoai:repisalud.isciii.es:20.500.12105/69682024-11-28T18:27:53Zcom_20.500.12105_2052com_20.500.12105_2051com_20.500.12105_15322col_20.500.12105_19609col_20.500.12105_16962
Repisalud
author
Domenech Lucas, Mirian
author
Damián, Diana
author
Ardanuy, Carmen
author
Liñares, Josefina
author
Fenoll, Asuncion
author
García, Ernesto
funder
Ministerio de Economía y Competitividad (España)
funder
Instituto de Salud Carlos III
2018-12-27T11:44:27Z
2018-12-27T11:44:27Z
2015-04-30
PLoS One. 2015 Apr 30;10(4):e0125636
10.1371/journal.pone.0125636
1932-6203
1932-6203
PloS one
25927917
http://hdl.handle.net/20.500.12105/6968
BACKGROUND: Since the use of pneumococcal conjugate vaccines PCV7 and PCV13 in children became widespread, invasive pneumococcal disease (IPD) has dramatically decreased. Nevertheless, there has been a rise in incidence of Streptococcus pneumoniae non-vaccine serotypes (NVT) colonising the human nasopharynx. Nasopharyngeal colonisation, an essential step in the development of S. pneumoniae-induced IPD, is associated with biofilm formation. Although the capsule is the main pneumococcal virulence factor, the formation of pneumococcal biofilms might, in fact, be limited by the presence of capsular polysaccharide (CPS). METHODOLOGY/PRINCIPAL FINDINGS: We used clinical isolates of 16 emerging, non-PCV13 serotypes as well as isogenic transformants of the same serotypes. The biofilm formation capacity of isogenic transformants expressing CPSs from NVT was evaluated in vitro to ascertain whether this trait can be used to predict the emergence of NVT. Fourteen out of 16 NVT analysed were not good biofilm formers, presumably because of the presence of CPS. In contrast, serotypes 11A and 35B formed ≥45% of the biofilm produced by the non-encapsulated M11 strain. CONCLUSIONS/SIGNIFICANCE: This study suggest that emerging, NVT serotypes 11A and 35B deserve a close surveillance.
eng
Emerging, Non-PCV13 Serotypes 11A and 35B of Streptococcus pneumoniae Show High Potential for Biofilm Formation In Vitro
journal article
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EmergingNon-PCV13Serotypes11A_2015.pdf
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https://repisalud.isciii.es/bitstreams/72e3b9f7-6749-46fc-a0d1-d3f6414f70d6/download
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EmergingNon-PCV13Serotypes11A_2015.pdf.txt