2026-06-14T03:31:41Zhttps://repisalud.isciii.es/rest/oai/requestoai:repisalud.isciii.es:20.500.12105/69442025-05-07T13:05:28Zcom_20.500.12105_2052com_20.500.12105_2051col_20.500.12105_19611col_20.500.12105_19609
Repisalud
author
Cuadros, Juan
author
Pérez-Tanoira, Ramón
author
Prieto-Pérez, Laura
author
Martin-Martin, Ines
author
Berzosa, Pedro
author
Gonzalez-Mora, Vicenta
author
Tisiano, Gebre
author
Balcha, Seble
author
Ramos, Jose Manuel
author
Górgolas, Miguel
funder
Rural Gambo Hospital (Etiopia)
funder
Autonomous University of Madrid (España)
2018-12-26T14:41:09Z
2018-12-26T14:41:09Z
2015-11-10
PLoS One. 2015 Nov 10;10(11):e0142842
10.1371/journal.pone.0142842
1932-6203
1932-6203
PloS one
26555068
http://hdl.handle.net/20.500.12105/6944
BACKGROUND: In up to one third of the hospitals in some rural areas of Africa, laboratory services in malaria diagnosis are limited to microscopy by thin film, as no capability to perform thick film exists (gold standard in terms of sensitivity for malaria diagnosis). A new rapid molecular malaria diagnostic test called Loop-mediated isothermal DNA amplification (LAMP) has been recently validated in clinical trials showing exceptional sensitivity and specificity features. It could be a reliable diagnostic tool to be implemented without special equipment or training. OBJECTIVE: The objective of this proof of concept study was to confirm the feasibility of using LAMP technique for diagnosis of malaria in a rural Ethiopian hospital with limited resources. METHODOLOGY/PRINCIPAL FINDINGS: This study was carried out in Gambo General Hospital, West Arsi Province (Ethiopia), from November 1st to December 31st 2013. A total of 162 patients with a non-focal febrile syndrome were investigated. The diagnostic capability (sensitivity, specificity, positive predictive and negative predictive values) of rapid malaria tests and microscopy by thin film was evaluated in comparison with LAMP. Eleven (6.79%) out of the 162 patients with fever and suspected malaria, tested positive for LAMP, 3 (1.85%) for rapid malaria tests and none of the eleven cases was detected by thin film microscopy. CONCLUSIONS/SIGNIFICANCE: LAMP can be performed in basic rural laboratories without the need for specialized infrastructure and it may set a reliable tool for malaria control to detect a low level parasitemia.
eng
Field Evaluation of Malaria Microscopy, Rapid Malaria Tests and Loop-Mediated Isothermal Amplification in a Rural Hospital in South Western Ethiopia
research article
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URL
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FieldEvaluationOfMalaria_2015.pdf
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https://repisalud.isciii.es/bitstreams/7c54ca30-9341-4e19-9c55-15e406b80f32/download
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FieldEvaluationOfMalaria_2015.pdf.txt