2026-04-28T21:35:22Zhttps://repisalud.isciii.es/rest/oai/requestoai:repisalud.isciii.es:20.500.12105/69232025-02-27T14:33:40Zcom_20.500.12105_2052com_20.500.12105_2051col_20.500.12105_19617
Repisalud
author
Bodega, Guillermo
author
Segura-Collar, Berta
author
Ciordia, Sergio
author
Mena, María Del Carmen
author
López-Fernández, Luis Andrés
author
García, María Isabel
author
Trabado, Isabel
author
Suárez, Isabel
funder
University of Alcalá (España)
2018-12-21T09:50:39Z
2018-12-21T09:50:39Z
2015-09-30
PLoS One. 2015 Sep 30;10(9):e0139619
1932-6203
http://hdl.handle.net/20.500.12105/6923
26421615
10.1371/journal.pone.0139619
1932-6203
PloS one
Primary cultures of rat astroglial cells were exposed to 1, 3 and 5 mM NH4Cl for up to 10 days. Dose- and time-dependent reductions in cell numbers were seen, plus an increase in the proportion of cells in the S phase. The DNA content was reduced in the treated cells, and BrdU incorporation diminished. However, neither ammonia nor ammonia plus glutamine had any effect on DNA polymerase activity. iTRAQ analysis showed that exposure to ammonia induced a significant reduction in histone and heterochromatin protein 1 expression. A reduction in cell viability was also noted. The ammonia-induced reduction of proliferative activity in these cultured astroglial cells seems to be due to a delay in the completion of the S phase provoked by the inhibition of chromatin protein synthesis.
eng
Ammonia Affects Astroglial Proliferation in Culture
research article
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https://repisalud.isciii.es/bitstreams/0ec084c6-54d9-4e8d-b156-eb74fd956425/download
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AmmoniaAffectsAstroglialProliferation_2015.pdf
URL
https://repisalud.isciii.es/bitstreams/30d1ab78-7eda-4de5-8974-b2efcfb80664/download
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text/plain
AmmoniaAffectsAstroglialProliferation_2015.pdf.txt