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00925njm 22002777a 4500 dc Bodega, Guillermo author Segura-Collar, Berta author Ciordia, Sergio author Mena, María Del Carmen author López-Fernández, Luis Andrés author García, María Isabel author Trabado, Isabel author Suárez, Isabel author 2015-09-30 Primary cultures of rat astroglial cells were exposed to 1, 3 and 5 mM NH4Cl for up to 10 days. Dose- and time-dependent reductions in cell numbers were seen, plus an increase in the proportion of cells in the S phase. The DNA content was reduced in the treated cells, and BrdU incorporation diminished. However, neither ammonia nor ammonia plus glutamine had any effect on DNA polymerase activity. iTRAQ analysis showed that exposure to ammonia induced a significant reduction in histone and heterochromatin protein 1 expression. A reduction in cell viability was also noted. The ammonia-induced reduction of proliferative activity in these cultured astroglial cells seems to be due to a delay in the completion of the S phase provoked by the inhibition of chromatin protein synthesis. PLoS One. 2015 Sep 30;10(9):e0139619 1932-6203 http://hdl.handle.net/20.500.12105/6923 26421615 10.1371/journal.pone.0139619 1932-6203 PloS one Ammonia Affects Astroglial Proliferation in Culture