2026-05-18T13:49:26Zhttps://repisalud.isciii.es/rest/oai/request

oai:repisalud.isciii.es:20.500.12105/69182025-06-09T11:11:01Zcom_20.500.12105_15322com_20.500.12105_2051com_20.500.12105_2052col_20.500.12105_16984col_20.500.12105_19616

00925njm 22002777a 4500 dc Peña-Chilet, Maria author Martínez, Maria T author Pérez-Fidalgo, Jose Alejandro author Peiró-Chova L author Oltra, Sara S author Tormo, Eduardo author Alonso-Yuste, Elisa author Martinez-Delgado, Beatriz author Eroles, Pilar author Climent, Joan author Burgués, Octavio author Ferrer-Lozano, Jaime author Bosch, Ana author Lluch, Ana author Ribas, Gloria author 2014-07-21 BACKGROUND: Breast cancer is rarely diagnosed in very young women (35 years old or younger), and it often presents with distinct clinical-pathological features related to a more aggressive phenotype and worse prognosis when diagnosed at this early age. A pending question is whether breast cancer in very young women arises from the deregulation of different underlying mechanisms, something that will make this disease an entity differentiated from breast cancer diagnosed in older patients. METHODS: We performed a comprehensive study of miRNA expression using miRNA Affymetrix2.0 array on paraffin-embedded tumour tissue of 42 breast cancer patients 35 years old or younger, 17 patients between 45 and 65 years old and 29 older than 65 years. Data were statistically analyzed by t-test and a hierarchical clustering via average linkage method was conducted. Results were validated by qRT-PCR. Putative targeted pathways were obtained using DIANA miRPath online software. RESULTS: The results show a differential and unique miRNA expression profile of 121 miRNAs (p-value <0.05), 96 of those with a FDR-value <0.05. Hierarchical clustering grouped the samples according to their age, but not by subtype nor by tumour characteristics. We were able to validate by qRT-PCR differences in the expression of 6 miRNAs: miR-1228*, miR-3196, miR-1275, miR-92b, miR-139 and miR-1207. Moreover, all of the miRNAs maintained the expression trend. The validated miRNAs pointed out pathways related to cell motility, invasion and proliferation. CONCLUSIONS: The study suggests that breast cancer in very young women appears as a distinct molecular signature. To our knowledge, this is the first time that a validated microRNA profile, distinctive to breast cancer in very young women, has been presented. The miRNA signature may be relevant to open an important field of research in order to elucidate the underlying mechanism in this particular disease, which in a more clinical setting, could potentially help to identify therapeutic targets in this particular set of patients. BMC Cancer. 2014 Jul 21;14:529. 10.1186/1471-2407-14-529 1471-2407 BMC cancer 25047087 http://hdl.handle.net/20.500.12105/6918 Breast cancer Young women miRNA Molecular profile MicroRNA profile in very young women with breast cancer