2026-06-14T17:49:23Zhttps://repisalud.isciii.es/rest/oai/requestoai:repisalud.isciii.es:20.500.12105/68992025-06-12T13:40:18Zcom_20.500.12105_2052com_20.500.12105_2051col_20.500.12105_19609
Repisalud
author
Gottstein, Bruno
author
Schneeberger, Marianne
author
Boubaker, Ghalia
author
Merkle, Bernadette
author
Huber, Cristina
author
Spiliotis, Markus
author
Müller, Norbert
author
Garate, Teresa
author
Doherr, Marcus G
2018-12-19T10:44:33Z
2018-12-19T10:44:33Z
2014-06-12
PLoS Negl Trop Dis. 2014 Jun 12;8(6):e2860
10.1371/journal.pntd.0002860
1935-2735
1935-2735
PLoS neglected tropical diseases
24922050
http://hdl.handle.net/20.500.12105/6899
Two recombinant Fasciola hepatica antigens, saposin-like protein-2 (recSAP2) and cathepsin L-1 (recCL1), were assessed individually and in combination in enzyme-linked immunosorbent assays (ELISA) for the specific serodiagnosis of human fasciolosis in areas of low endemicity as encountered in Central Europe. Antibody detection was conducted using ProteinA/ProteinG (PAG) conjugated to alkaline phosphatase. Test characteristics as well as agreement with results from an ELISA using excretory-secretory products (FhES) from adult stage liver flukes was assessed by receiver operator characteristic (ROC) analysis, specificity, sensitivity, Youdens J and overall accuracy. Cross-reactivity was assessed using three different groups of serum samples from healthy individuals (n=20), patients with other parasitic infections (n=87) and patients with malignancies (n=121). The best combined diagnostic results for recombinant antigens were obtained using the recSAP2-ELISA (87% sensitivity, 99% specificity and 97% overall accuracy) employing the threshold (cut-off) to discriminate between positive and negative reactions that maximized Youdens J. The findings showed that recSAP2-ELISA can be used for the routine serodiagnosis of chronic fasciolosis in clinical laboratories; the use of the PAG-conjugate offers the opportunity to employ, for example, rabbit hyperimmune serum for the standardization of positive controls.
eng
Comparative assessment of ELISAs using recombinant saposin-like protein 2 and recombinant cathepsin L-1 from Fasciola hepatica for the serodiagnosis of human Fasciolosis
research article
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URL
https://repisalud.isciii.es/bitstreams/f89d09f5-3b26-41e7-98b4-97452bb2a163/download
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ComparativeAssessmentOfELISAs_2014.pdf
URL
https://repisalud.isciii.es/bitstreams/aa65d093-299e-446b-8e35-8a9cfedabdf0/download
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text/plain
ComparativeAssessmentOfELISAs_2014.pdf.txt