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00925njm 22002777a 4500 dc Wong Sak Hoi, Joanne author Lamarre, Claude author Beau, Rémi author Meneau, Isabelle author Berepiki, Adokiye author Barre, Annick author Mellado, Emilia author Read, Nick D author Latgé, Jean-Paul author 2011-06-01 During a search for genes controlling conidial dormancy in Aspergillus fumigatus, two dehydrin-like genes, DprA and DprB, were identified. The deduced proteins had repeated stretches of 23 amino acids that contained a conserved dehydrin-like protein (DPR) motif. Disrupted DprAΔ mutants were hypersensitive to oxidative stress and to phagocytic killing, whereas DprBΔ mutants were impaired in osmotic and pH stress responses. However, no effect was observed on their pathogenicity in our experimental models of invasive aspergillosis. Molecular dissection of the signaling pathways acting upstream showed that expression of DprA was dependent on the stress-activated kinase SakA and the cyclic AMP-protein kinase A (cAMP-PKA) pathways, which activate the bZIP transcription factor AtfA, while expression of DprB was dependent on the SakA mitogen-activated protein kinase (MAPK) pathway, and the zinc finger transcription factor PacC. Fluorescent protein fusions showed that both proteins were associated with peroxisomes and the cytosol. Accordingly, DprA and DprB were important for peroxisome function. Our findings reveal a novel family of stress-protective proteins in A. fumigatus and, potentially, in filamentous ascomycetes. Mol Biol Cell. 2011;22(11):1896-906. 10.1091/mbc.E10-11-0914 1939-4586 Molecular biology of the cell 21490150 http://hdl.handle.net/20.500.12105/6881 A novel family of dehydrin-like proteins is involved in stress response in the human fungal pathogen Aspergillus fumigatus