2026-06-14T06:44:08Zhttps://repisalud.isciii.es/rest/oai/requestoai:repisalud.isciii.es:20.500.12105/68792025-05-14T10:11:17Zcom_20.500.12105_2052com_20.500.12105_2051com_20.500.12105_15322col_20.500.12105_19609col_20.500.12105_16961
Repisalud
author
Gonzalez-Fernandez, Nuria
author
McKee, Krisha
author
Lynch, Rebecca M
author
Georgiev, Ivelin S
author
Jiménez, Laura
author
Grau, Eulalia
author
Yuste-Herranz, Maria Eloisa
author
Kwong, Peter D
author
Mascola, John R
author
Alcamí, José
funder
Instituto de Salud Carlos III
funder
Fundación para la Investigación y la Prevención del Sida en España
funder
Unión Europea. Fondo Europeo de Desarrollo Regional (FEDER/ERDF)
2018-12-17T14:23:22Z
2018-12-17T14:23:22Z
2018-03-20
PLoS One. 2018 Mar 20;13(3):e0193773.
10.1371/journal.pone.0193773
1932-6203
1932-6203
PloS one
29558468
http://hdl.handle.net/20.500.12105/6879
BACKGROUND: Only a small fraction of HIV-1-infected patients develop broadly neutralizing antibodies (bNAbs), a process generally associated to chronic antigen stimulation. It has been described that rare aviremic HIV-1-infected patients can generate bNAbs but this issue remains controversial. To address this matter we have assessed bNAb responses in a large cohort of long-term non-progressors (LTNPs) with low or undetectable viremia. METHODS: Samples from the LTNP cohort of the Spanish AIDS Research Network (87 elite and 42 viremic controllers) and a control population of 176 viremic typical-progressors (TPs) were screened for bNAbs using Env-recombinant viruses. bNAb specificities were studied by ELISA using mutated gp120, neutralization assays with mutated viruses, and peptide competition. Epitope specificities were also elucidated from the serum pattern of neutralization against a panel of diverse HIV-1 isolates. RESULTS: Broadly neutralizing sera were found among 9.3% LTNPs, both elite (7%) and viremic controllers (14%). Within the broadly neutralizing sera, CD4 binding site antibodies were detected by ELISA in 4/12 LTNPs (33%), and 16/33 of TPs (48%). Anti-MPER antibodies were detected in 6/12 LTNPs (50%) and 14/33 TPs (42%) whereas glycan-dependent HIV-1 bNAbs were more frequent in LTNPs (11/12, 92%) as compared to TPs (12/33, 36%). A good concordance between standard serum mapping and neutralization-based mapping was observed. CONCLUSION: LTNPs, both viremic and elite controllers, showed broad humoral immune responses against HIV-1, including activity against many major epitopes involved in bNAbs-mediated protection.
eng
Characterization of broadly neutralizing antibody responses to HIV-1 in a cohort of long term non-progressors
research article
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https://repisalud.isciii.es/bitstreams/a40edb0b-5952-4241-856b-6a65aedcdb1b/download
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c97d89ff2207ef233319cb494a94e95b
3805030
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CharacterizationOfBroadlyNeutralizing_2018.pdf
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https://repisalud.isciii.es/bitstreams/1b75d17a-bd2a-4856-8c1d-ecc2ec6aae21/download
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9ec97d47d647a91ce6814e5d5aba74f2
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text/plain
CharacterizationOfBroadlyNeutralizing_2018.pdf.txt